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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 17 — Aug. 18, 2008
  • pp: 12640–12649

Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber

Yu-Chung Chang, Jing Yong Ye, Thommey Thomas, Yi Chen, James R. Baker, Jr., and Theodore B. Norris  »View Author Affiliations

Optics Express, Vol. 16, Issue 17, pp. 12640-12649 (2008)

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We report on the utilization of a dual-clad optical fiber for two-photon excited fluorescence correlation spectroscopy (FCS). High excitation efficiency is obtained by sending prechirped ultrafast pulses through the single-mode core of the fiber, while the fluorescence collection efficiency is enhanced because of the larger numerical aperture of the inner cladding. We show that the utilization of a dual-clad fiber is critical for ultrasensitive two-photon fluorescence detection. Our system has the ability to detect fluorescent nanospheres as small as 12 nm in radius. Quantum dots of radius 7 nm are also measured and show excellent signal to noise ratio. The particle sizes obtained from the fiber FCS system were confirmed by measurements using a commercial dynamic light scattering (DLS) system.

© 2008 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4180) Nonlinear optics : Multiphoton processes
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:

Original Manuscript: May 22, 2008
Revised Manuscript: August 2, 2008
Manuscript Accepted: August 4, 2008
Published: August 6, 2008

Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics

Yu-Chung Chang, Jing Yong Ye, Thommey Thomas, Yi Chen, James R. Baker, and Theodore B. Norris, "Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber," Opt. Express 16, 12640-12649 (2008)

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