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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 17 — Aug. 18, 2008
  • pp: 12737–12745

Intrinsic Raman spectroscopy for quantitative biological spectroscopy Part II: Experimental applications

Kate L. Bechtel, Wei-Chuan Shih, and Michael S. Feld  »View Author Affiliations


Optics Express, Vol. 16, Issue 17, pp. 12737-12745 (2008)
http://dx.doi.org/10.1364/OE.16.012737


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Abstract

We demonstrate the effectiveness of intrinsic Raman spectroscopy (IRS) at reducing errors caused by absorption and scattering. Physical tissue models, solutions of varying absorption and scattering coefficients with known concentrations of Raman scatterers, are studied. We show significant improvement in prediction error by implementing IRS to predict concentrations of Raman scatterers using both ordinary least squares regression (OLS) and partial least squares regression (PLS). In particular, we show that IRS provides a robust calibration model that does not increase in error when applied to samples with optical properties outside the range of calibration.

© 2008 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.5280) Medical optics and biotechnology : Photon migration
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 27, 2008
Revised Manuscript: July 19, 2008
Manuscript Accepted: July 24, 2008
Published: August 7, 2008

Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Kate L. Bechtel, Wei-Chuan Shih, and Michael S. Feld, "Intrinsic Raman spectroscopy for quantitative biological spectroscopy Part II: Experimental applications," Opt. Express 16, 12737-12745 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-17-12737


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