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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 18 — Sep. 1, 2008
  • pp: 13624–13636

Dual-beam Fourier domain optical Doppler tomography of zebrafish

Nicusor V. Iftimia, Daniel X. Hammer, R. Daniel Ferguson, Mircea Mujat, Danthu Vu, and Anthony A. Ferrante  »View Author Affiliations

Optics Express, Vol. 16, Issue 18, pp. 13624-13636 (2008)

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We have developed a dual-beam Fourier domain optical Doppler tomography (FD-ODT) system to image zebrafish (Danio rerio) larvae. Two beams incident on the zebrafish with a fixed angular separation allow absolute blood flow velocity measurement to be made regardless of vessel orientation in a sagittal plane along which the heart and most of the major vasculature lie. Two spectrometers simultaneously acquire spectra from two interferometers with a typical (maximum) line rate of 18 (28) kHz. The system was calibrated using diluted milk and microspheres and a 0.5-mm thick flow cell. The average deviation from the set velocity from 1.4 to 34.6 mm/s was 4.1%. Three-dimensional structural raster videos were acquired of an entire fish, and through the head, heart, and upper tail of the fish. Coarse features that were resolved include the telencephalon, retina, both heart chambers (atrium and ventricle), branchial arches, and notochord. Other fine structures within these organs were also resolved. Zebrafish are an important tool for high-throughput screening of new pharmacological agents. The ability to generate high-resolution three-dimensional structural videos and accurately measure absolute flow rates in major vessels with FD-ODT provides researchers with additional metrics by which the efficacy of new drugs can be assessed.

© 2008 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 2, 2008
Revised Manuscript: August 4, 2008
Manuscript Accepted: August 4, 2008
Published: August 20, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Nicusor V. Iftimia, Daniel X. Hammer, R. D. Ferguson, Mircea Mujat, Danthu Vu, and Anthony A. Ferrante, "Dual-beam Fourier domain optical Doppler tomography of zebrafish," Opt. Express 16, 13624-13636 (2008)

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