OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 19 — Sep. 15, 2008
  • pp: 14561–14570

Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy

Graham M Gibson, Jonathan Leach, Stephen Keen, Amanda J Wright, and Miles J Padgett  »View Author Affiliations

Optics Express, Vol. 16, Issue 19, pp. 14561-14570 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (243 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We assess the performance of a CMOS camera for the measurement of particle position within optical tweezers and the associated autocorrelation function and power spectrum. Measurement of the displacement of the particle from the trap center can also be related to the applied force. By considering the Allan variance of these measurements, we show that such cameras are capable of reaching the thermal limits of nanometer and femtonewton accuracies, and hence are suitable for many of the applications that traditionally use quadrant photodiodes. As an example of a multi-particle measurement we show the hydrodynamic coupling between two particles.

© 2008 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: June 27, 2008
Revised Manuscript: August 13, 2008
Manuscript Accepted: August 25, 2008
Published: September 2, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Graham M. Gibson, Jonathan Leach, Stephen Keen, Amanda J. Wright, and Miles J. Padgett, "Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy," Opt. Express 16, 14561-14570 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, and S. Chu, "Observation of a single-beam gradient force optical trap for dielectric particles," Opt. Lett. 11, 288-290 (1986). [CrossRef] [PubMed]
  2. J. E. Molloy and M. J. Padgett, "Lights, action: optical tweezers," Contemp. Phys. 43, 241-258 (2002). [CrossRef]
  3. J.-C. Meiners and S. R. Quake, "Femtonewton force spectroscopy of single extended DNA molecules," Phys. Rev. Lett. 84, 5014-5017 (2000). [CrossRef] [PubMed]
  4. K. C. Neuman and S. M. Block, "Optical trapping," Rev. Sci. Instrum. 75, 2787-2809 (2004). [CrossRef]
  5. M. Polin, D. G. Grier, and S. R. Quake, "Anomalous vibrational dispersion in holographically trapped colloidal arrays," Phys. Rev. Lett. 96, 088101 (2006). [CrossRef] [PubMed]
  6. S. Keen, J. Leach, G. Gibson, and M. Padgett, "Comparison of a high-speed camera and a quadrant detector for measuring displacements in optical tweezers," J. Opt. A: Pure Appl. Opt. 9, S264-S266 (2007). [CrossRef]
  7. O. Otto, C. Gutsche, F. Kremer, and U. F. Keyser, "Optical tweezers with 2.5kHz bandwidth video detection for single-colloid electrophoresis," Rev. Sci. Instrum. 79, 023710 (2008). [CrossRef] [PubMed]
  8. D. W. Allan, "Statistics of atomic frequency standards," Proc. IEEE 54, 221-230 (1966). [CrossRef]
  9. J. Leach, K. Wulff, G. Sinclair, P. Jordan, J. Courtial, L. Thomson, G. Gibson, K. Karunwi, J. Cooper, Z. J. Laczik, and M. Padgett, "Interactive approach to optical tweezers control," Appl. Opt. 45, 897-903 (2006). [CrossRef] [PubMed]
  10. G. Gibson, D. M. Carberry, G. Whyte, J. Leach, J. Courtial, J. C. Jackson, D. Robert, M. Miles, and M. Padgett, "Holographic assembly workstation for optical manipulation," J. Opt. A: Pure Appl. Opt. 10, 044009 (2008). [CrossRef]
  11. K. Berg-Sørensen and H. Flyvbjerg, "Power spectrum analysis for optical tweezers," Rev. Sci. Instrum. 75, 594-612 (2004). [CrossRef]
  12. J. R. Moffitt, Y. R. Chemla, D. Izhaky, and C. Bustamante, "Differential detection of dual traps improves the spatial resolution of optical tweezers," PNAS 103, 9006-9011 (2006). [CrossRef] [PubMed]
  13. M. Klein, M. Andersson, O. Axner, and E. F¨allman, "Dual-trap technique for reduction of low-frequency noise in force measuring optical tweezers," Appl. Opt. 46, 405-412 (2007). [CrossRef] [PubMed]
  14. M. Atakhorrami, K. M. Addas, and C. F. Schmidt, "Twin optical traps for two-particle cross-correlation measurements: Eliminating cross-talk," Rev. Sci. Instrum. 79, 043103 (2008). [CrossRef] [PubMed]
  15. J.-C. Meiners and S. R. Quake, "Direct measurement of hydrodynamic cross correlations between two particles in an external potential," Phys. Rev. Lett. 82, 2211-2214 (1999). [CrossRef]
  16. C. D. Saunter, G. D. Love,M. Johns, and J. Holmes, "FGPA technology for high-speed, low-cost adaptive optics," vol. 6018 of 5th International Workshop on Adaptive Optics for Industry and Medicine (SPIE, 2005).
  17. R. Di Leonardo, S. Keen, J. Leach, C. D. Saunter, G. D. Love, G. Ruocco, and M. J. Padgett, "Eigenmodes of a hydrodynamically coupled micron-size multiple-particle ring," Phys. Rev. E 76, 061402 (2007). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited