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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 19 — Sep. 15, 2008
  • pp: 14561–14570

Optics InfoBase > Optics Express > Volume 16 > Issue 19 > Page 14561

Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy

Graham M. Gibson, Jonathan Leach, Stephen Keen, Amanda J. Wright, and Miles J. Padgett  »View Author Affiliations


Optics Express, Vol. 16, Issue 19, pp. 14561-14570 (2008)
http://dx.doi.org/10.1364/OE.16.014561


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Abstract

We assess the performance of a CMOS camera for the measurement of particle position within optical tweezers and the associated autocorrelation function and power spectrum. Measurement of the displacement of the particle from the trap center can also be related to the applied force. By considering the Allan variance of these measurements, we show that such cameras are capable of reaching the thermal limits of nanometer and femtonewton accuracies, and hence are suitable for many of the applications that traditionally use quadrant photodiodes. As an example of a multi-particle measurement we show the hydrodynamic coupling between two particles.

© 2008 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Trapping and Manipulation

History
Original Manuscript: June 27, 2008
Revised Manuscript: August 13, 2008
Manuscript Accepted: August 25, 2008
Published: September 2, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Graham M. Gibson, Jonathan Leach, Stephen Keen, Amanda J. Wright, and Miles J. Padgett, "Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy," Opt. Express 16, 14561-14570 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-19-14561


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References

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