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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 19 — Sep. 15, 2008
  • pp: 14703–14715

Third-harmonic generation microscopy with focus-engineered beams: a numerical study

Nicolas Olivier and Emmanuel Beaurepaire  »View Author Affiliations

Optics Express, Vol. 16, Issue 19, pp. 14703-14715 (2008)

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We use a vector field model to analyze third-harmonic generation (THG) from model geometries (interfaces, slabs, periodic structures) illuminated by Hermite-Gaussian (HG) and Laguerre-Gaussian (LG) beams focused by a high NA lens. Calculations show that phase matching conditions are significantly affected by the tailoring of the field distribution near focus. In the case of an interface parallel to the optical axis illuminated by an odd HG mode, the emission patterns and signal level reflect the relative orientation of the interface and the focal field structure. In the case of slabs and periodic structures, the emission patterns reflect the interplay between focal field distribution (amplitude and phase) and sample structure. Forward-to-backward emission ratios using different beam shapes provide sub-wavelength information about sample spatial frequencies.

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation

ToC Category:

Original Manuscript: July 8, 2008
Revised Manuscript: August 21, 2008
Manuscript Accepted: August 21, 2008
Published: September 3, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Nicolas Olivier and Emmanuel Beaurepaire, "Third-harmonic generation microscopy with focus-engineered beams: a numerical study," Opt. Express 16, 14703-14715 (2008)

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  1. E. Yew and C. Sheppard, "Second harmonic generation microscopy with tightly focused linearly and radially polarized beams," Opt. Commun. 275, 453-457 (2007). [CrossRef]
  2. K. Yoshiki, R. Kanamaru, M. Hashimoto, N. Hashimoto, and T. Araki, "Second-harmonic-generation microscope using eight-segment polarization-mode converter to observe three-dimensional molecular orientation," Opt. Lett. 32, 1680-1682 (2007). [CrossRef] [PubMed]
  3. V. V. Krishnamachari and E. O. Potma, "Focus-engineered coherent anti-Stokes Raman scattering microscopy: a numerical investigation," J. Opt. Soc. Am. A 24, 1138-1147 (2007). [CrossRef]
  4. V. V. Krishnamachari and E. O. Potma, "Imaging chemical interfaces perpendicular to the optical axis with focus-engineered coherent anti-Stokes Raman scattering microscopy," Chem. Phys. 341, 81-88 (2007). [CrossRef]
  5. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, "Nonlinear scanning laser microscopy by third harmonic generation," Appl. Phys. Lett. 70, 922-924 (1997). [CrossRef]
  6. M. M¨uller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, "3D-microscopy of transparent objects using thirdharmonic generation," J. Microsc. 191, 266-274 (1998). [CrossRef] [PubMed]
  7. D. Débarre and E. Beaurepaire, "Quantitative characterization of biological liquids for third-harmonic generation microscopy," Biophys. J. 92, 603-612 (2007). [CrossRef]
  8. D. Yelin and Y. Silberberg, "Laser scanning third-harmonic generation microscopy in biology," Opt. Express 5 (1999). [CrossRef] [PubMed]
  9. D. Oron, D. Yelin, E. Tal, S. Raz, R. Fachima, and Y. Silberberg, "Depth-resolved structural imaging by thirdharmonic generation microscopy," J. Struct. Biol. 147, 3-11 (2004). [CrossRef] [PubMed]
  10. D. Débarre, W. Supatto, E. Farge, B. Moulia, M.-C. Schanne-Klein, and E. Beaurepaire, "Velocimetric thirdharmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos," Opt. Lett. 29, 2881-2883 (2004). [CrossRef]
  11. C.-K. Sun, S.-W. Chu, S.-Y. Chen, T.-H. Tsai, T.-M. Liu, C.-Y. Lin, and H.-J. Tsai, "Higher harmonic generation microscopy for developmental biology," J. Struct. Biol. 147, 19-30 (2004). [CrossRef] [PubMed]
  12. W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J.-L. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses," Proc. Nat. Acad. Sci. USA 102, 1047-1052 (2005). [CrossRef] [PubMed]
  13. D. Débarre, W. Supatto, A.-M. Pena, A. Fabre, T. Tordjmann, L. Combettes, M.-C. Schanne-Klein, and E. Beaurepaire, "Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy," Nat. Methods 3, 47-53 (2006). [CrossRef]
  14. D. Débarre, W. Supatto, and E. Beaurepaire, "Structure sensitivity in third-harmonic generation microscopy," Opt. Lett. 30, 2134-2136 (2005). [CrossRef] [PubMed]
  15. J.-X. Cheng and X. S. Xie, "Green???s function formulation for third harmonic generation microscopy," J. Opt. Soc. Am. B 19, 1604-1610 (2002). [CrossRef]
  16. B. Richards and E. Wolf, "Electromagnetic diffraction in optical systems II. Structure of the image field in an aplanetic system.," Proc. Royal Soc. A 253, 358-379 (1959). [CrossRef]
  17. L. Novotny and B. Hecht, Principles of nano-optics (Cambridge Univ Press, 2006).
  18. Boyd, R. W. Nonlinear optics, 2nd edition, (Academic Press 2003).
  19. H. Kogelnik and T. Li, Laser beams and resonators," Appl. Opt. 5, 1550 (1966). [CrossRef] [PubMed]
  20. K. Youngworth and T. Brown, "Focusing of high numerical aperture cylindrical-vector beams," Opt. Express 7, 77-87 (2000). [CrossRef] [PubMed]
  21. D. Débarre, N. Olivier, and E. Beaurepaire, "Signal epidetection in third-harmonic generation microscopy of turbid media," Opt. Express 15, 8913-8924 (2007). [CrossRef] [PubMed]
  22. E. Y. S. Yew and C. J. R. Sheppard, "Fractional Gouy phase," Opt. Lett. 33, 1363-1365 (2008). [CrossRef] [PubMed]
  23. J. Mertz and L. Moreaux, "Second-harmonic generation by focused excitation of inhomogeneously distributed scatterers," Opt. Commun. 196, 325-330 (2001). [CrossRef]
  24. C. J. R. Sheppard, "High-aperture beams," J. Opt. Soc. Am. A 18, 1579-1587 (2001). [CrossRef]
  25. S. Quabis, R. Dorn, M. Eberler, O. Glöckl, and G. Leuchs, "Focusing light to a tighter spot," Opt. Commun. 179, 1-7 (2000). [CrossRef]
  26. S. Carrasco, B. E. A. Saleh, M. C. Teich, and J. T. Fourkas, "Second- and third-harmonic generation with vector Gaussian beams," J. Opt. Soc. Am. B 23, 2134-2141 (2006). [CrossRef]
  27. S. S. Sherif, M. R. Foreman, and P. Török, "Eigenfunction expansion of the electric fields in the focal region of a high numerical aperture focusing system," Opt. Express 16, 3397-3407 (2008). [CrossRef] [PubMed]
  28. M. R. Foreman, S. S. Sherif, P. R. T. Munro, and P. Török, "Inversion of the Debye-Wolf diffraction integral using an eigenfunction representation of the electric fields in the focal region," Opt. Express 16, 4901-4917 (2008). [CrossRef] [PubMed]

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