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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 19 — Sep. 15, 2008
  • pp: 14910–14921

Optical recording of electrical activity in intact neuronal networks with random access second-harmonic generation microscopy

Leonardo Sacconi, Jonathan Mapelli, Daniela Gandolfi, Jacopo Lotti, Rodney P. O’Connor, Egidio D’Angelo, and Francesco S. Pavone  »View Author Affiliations

Optics Express, Vol. 16, Issue 19, pp. 14910-14921 (2008)

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One of the main challenges in understanding the central nervous system is to measure the network dynamics of neuronal assemblies, while preserving the computational role of individual neurons. However, this is not possible with current techniques. In this work, we combined the advantages of second-harmonic generation (SHG) with a random access (RA) excitation scheme to realize a new microscope (RASH) capable of optically recording fast membrane potential events occurring in a wide-field of view. The RASH microscope, in combination with bulk loading of tissue with FM4-64 dye, was used to simultaneously record electrical activity from clusters of Purkinje cells in acute cerebellar slices. Complex spikes, both synchronous and asynchronous, were optically recorded simultaneously across a given population of neurons. Spontaneous electrical activity was also monitored simultaneously in pairs of neurons, where action potentials were recorded without averaging across trials. These results show the strength of this technique in describing the temporal dynamics of neuronal assemblies, opening promising perspectives in understanding the computations of neuronal networks.

© 2008 Optical Society of America

OCIS Codes
(170.1420) Medical optics and biotechnology : Biology
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: July 24, 2008
Revised Manuscript: September 3, 2008
Manuscript Accepted: September 3, 2008
Published: September 8, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Leonardo Sacconi, Jonathan Mapelli, Daniela Gandolfi, Jacopo Lotti, Rodney P. O'Connor, Egidio D'Angelo, and Francesco S. Pavone, "Optical recording of electrical activity in intact neuronal networks with random access second-harmonic generation microscopy," Opt. Express 16, 14910-14921 (2008)

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