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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 19 — Sep. 15, 2008
  • pp: 15297–15303

Strong electromagnetic confinement near dielectric microspheres to enhance single-molecule fluorescence

Davy Gérard, Jérôme Wenger, Alexis Devilez, David Gachet, Brian Stout, Nicolas Bonod, Evgeny Popov, and Hervé Rigneault  »View Author Affiliations


Optics Express, Vol. 16, Issue 19, pp. 15297-15303 (2008)
http://dx.doi.org/10.1364/OE.16.015297


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Abstract

Latex microspheres are used as a simple and low-cost means to achieve three axis electromagnetic confinement below the standard diffraction limit. We demonstrate their use to enhance the fluorescence fluctuation detection of single molecules. Compared to confocal microscopy with high numerical aperture, we monitor a detection volume reduction of one order of magnitude below the diffraction limit together with a 5-fold gain in the fluorescence rate per molecule. This offers new opportunities for a broad range of applications in biophotonics, plasmonics, optical data storage and ultramicroscopy.

© 2008 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.0180) Microscopy : Microscopy
(230.3990) Optical devices : Micro-optical devices
(290.4020) Scattering : Mie theory

ToC Category:
Microscopy

History
Original Manuscript: August 21, 2008
Revised Manuscript: September 10, 2008
Manuscript Accepted: September 10, 2008
Published: September 12, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Davy Gérard, Jérôme Wenger, Alexis Devilez, David Gachet, Brian Stout, Nicolas Bonod, Evgeny Popov, and Hervé Rigneault, "Strong electromagnetic confinement near dielectric microspheres to enhance single-molecule fluorescence," Opt. Express 16, 15297-15303 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-19-15297


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References

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