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Optics Express

Optics Express

  • Editor: Martijn de Sterke
  • Vol. 16, Iss. 20 — Sep. 29, 2008
  • pp: 15640–15654

An optimal permissible source region strategy for multispectral bioluminescence tomography

Jinchao Feng, Kebin Jia, Guorui Yan, Shouping Zhu, Chenghu Qin, Yujie Lv, and Jie Tian  »View Author Affiliations

Optics Express, Vol. 16, Issue 20, pp. 15640-15654 (2008)

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Multispectral bioluminescence tomography (BLT) attracts increasing more attention in the area of small animal studies because multispectral data acquisition could help in the 3D location of bioluminescent sources. Generally, BLT problem is ill-posed and a priori information is indispensable to reconstruction bioluminescent source uniquely and quantitatively. In this paper, we propose a spectrally solved bioluminescence tomography algorithm with an optimal permissible source region strategy. Being the most different from earlier studies, an optimal permissible source region strategy which is automatically selected without human intervention is developed to reduce the ill-posedness of BLT and therefore improves the reconstruction quality. Furthermore, both numerical stability and computational efficiency benefit from the strategy. In the numerical experiments, a heterogeneous phantom is used to evaluate the proposed algorithm and the synthetic data is produced by Monte Carlo method for avoiding the inverse crime. The results demonstrate the feasibility and potential of our methodology for reconstructing the distribution of bioluminescent sources.

© 2008 Optical Society of America

OCIS Codes
(110.6960) Imaging systems : Tomography
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 16, 2008
Revised Manuscript: May 26, 2008
Manuscript Accepted: September 3, 2008
Published: September 19, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Jinchao Feng, Kebin Jia, Guorui Yan, Shouping Zhu, Chenghu Qin, Yujie Lv, and Jie Tian, "An optimal permissible source region strategy for multispectral bioluminescence tomography," Opt. Express 16, 15640-15654 (2008)

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