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Optics Express

Optics Express

  • Editor: Martijn de Sterke
  • Vol. 16, Iss. 20 — Sep. 29, 2008
  • pp: 16272–16283

Plastinated tissue samples as three-dimensional models for optical instrument characterization

Daniel L. Marks, Eric J. Chaney, and Stephen A. Boppart  »View Author Affiliations


Optics Express, Vol. 16, Issue 20, pp. 16272-16283 (2008)
http://dx.doi.org/10.1364/OE.16.016272


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Abstract

Histology of biological specimens is largely limited to investigating two-dimensional structure because of the sectioning required to produce optically thin samples for conventional microscopy. With the advent of three-dimensional optical imaging technologies such as optical coherence tomography (OCT), diffuse optical tomography (DOT), and multiphoton microscopy (MPM), methods of tissue preparation that minimally disrupt three-dimensional structure are needed. We propose plastination as a means of transforming tissues into three-dimensional models suitable for optical instrument characterization. Tissues are plastinated by infusing them with transparent polymers, after which they can be safely handled, unlike fresh or fixed tissues. Such models are useful for investigating threedimensional structure, testing and comparing the performance of optical instruments, and potentially investigating tissue properties not normally observed after the three-dimensional scattering properties of a biological samples are lost. We detail our plastination procedures and show examples of imaging several plastinated tissues from a pre-clinical rat model using optical coherence tomography.

© 2008 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(110.4500) Imaging systems : Optical coherence tomography
(120.4800) Instrumentation, measurement, and metrology : Optical standards and testing
(180.6900) Microscopy : Three-dimensional microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 4, 2008
Revised Manuscript: September 21, 2008
Manuscript Accepted: September 24, 2008
Published: September 26, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Daniel L. Marks, Eric J. Chaney, and Stephen A. Boppart, "Plastinated tissue samples as three-dimensional models for optical instrument characterization," Opt. Express 16, 16272-16283 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-20-16272


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