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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 21 — Oct. 13, 2008
  • pp: 16431–16441

Integrated multiple multi-photon imaging and Raman spectroscopy for characterizing structure-constituent correlation of tissues

Jhen-Wei Jhan, Wei-Tien Chang, Hung-Che Chen, Yu-Tsung Lee, Ming-Fang Wu, Chung-Hsuan Chen, and Ian Liau  »View Author Affiliations

Optics Express, Vol. 16, Issue 21, pp. 16431-16441 (2008)

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Histopathological imaging of tissues often requires extensive sample preparation including fixation and staining in order to highlight characteristic alterations associated with diseases. Herein, we report an integrated spectro-microscopy approach based on a combination of multi-modal multi-photon imaging and Raman micro-spectroscopy and demonstrate label-free characterization of the structure-constituent correlation of porcine skin. The multi-modal imaging allows the visualization of dermatological features whereas Raman micro-spectroscopy enables the identification of their ‘molecular fingerprints’. By obtaining both structural and molecular-level information of tissue constituents, this integrated approach can offer new insight into the patho-physiological status of tissues.

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5660) Medical optics and biotechnology : Raman spectroscopy
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: May 14, 2008
Revised Manuscript: September 19, 2008
Manuscript Accepted: September 24, 2008
Published: October 1, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Jhen-Wei Jhan, Wei-Tien Chang, Hung-Che Chen, Ming-Fang Wu, Yu-Tsung Lee, Chung-Hsuan Chen, and Ian Liau, "Integrated multiple multi-photon imaging and Raman spectroscopy for characterizing structure-constituent correlation of tissues," Opt. Express 16, 16431-16441 (2008)

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