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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 21 — Oct. 13, 2008
  • pp: 17031–17038

The divided aperture technique for microscopy through scattering media

Colin J. R. Sheppard, Wei Gong, and Ke Si  »View Author Affiliations


Optics Express, Vol. 16, Issue 21, pp. 17031-17038 (2008)
http://dx.doi.org/10.1364/OE.16.017031


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Abstract

A diffraction analysis is presented for image formation in confocal microscopy using the divided aperture technique, which uses two D-shaped apertures (also called specular microscopy). The effects of increasing the width of a divider, that separates the two D shapes, are investigated. As the width is increased, the resolution degrades. The efficiency of singly-scattered light rejection is not improved with increased width.

© 2008 Optical Society of America

OCIS Codes
(050.1220) Diffraction and gratings : Apertures
(170.0110) Medical optics and biotechnology : Imaging systems
(170.0180) Medical optics and biotechnology : Microscopy
(180.1790) Microscopy : Confocal microscopy
(260.1960) Physical optics : Diffraction theory

ToC Category:
Microscopy

History
Original Manuscript: August 6, 2008
Revised Manuscript: September 10, 2008
Manuscript Accepted: September 11, 2008
Published: October 10, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Colin J. Sheppard, Wei Gong, and Ke Si, "The divided aperture technique for microscopy through scattering media," Opt. Express 16, 17031-17038 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-21-17031


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