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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 22 — Oct. 27, 2008
  • pp: 17574–17584

Advancing multifocal nonlinear microscopy: development and application of a novel multibeam Yb:KGd(WO4)2 oscillator

Kraig E. Sheetz, Erich E. Hoover, Ramón Carriles, David Kleinfeld, and Jeff A. Squier  »View Author Affiliations


Optics Express, Vol. 16, Issue 22, pp. 17574-17584 (2008)
http://dx.doi.org/10.1364/OE.16.017574


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Abstract

We present a novel Yb:KGd(WO4)2 oscillator design that generates six beams of temporally delayed, 253 fs, 11 nJ pulses. This allows multifocal nonlinear microscopy to be performed without the need for complicated optical multiplexers. We demonstrate our design with twelve simultaneously acquired two-photon, second-harmonic and/or third-harmonic images generated from six laterally separated foci.

© 2008 Optical Society of America

OCIS Codes
(030.5260) Coherence and statistical optics : Photon counting
(140.3480) Lasers and laser optics : Lasers, diode-pumped
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.0180) Medical optics and biotechnology : Microscopy
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 5, 2008
Revised Manuscript: October 8, 2008
Manuscript Accepted: October 10, 2008
Published: October 16, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Kraig E. Sheetz, Erich E. Hoover, Ramn Carriles, David Kleinfeld, and Jeff A. Squier, "Advancing multifocal nonlinear microscopy: development and application of a novel multibeam Yb:KGd(WO4)2 oscillator," Opt. Express 16, 17574-17584 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-22-17574


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