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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 22 — Oct. 27, 2008
  • pp: 18215–18229

Quantitative interpretations of Visible-NIR reflectance spectra of blood

Yulia M. Serebrennikova, Jennifer M. Smith, Debra E. Huffman, German F. Leparc, and Luis H. García-Rubio  »View Author Affiliations

Optics Express, Vol. 16, Issue 22, pp. 18215-18229 (2008)

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This paper illustrates the implementation of a new theoretical model for rapid quantitative analysis of the Vis-NIR diffuse reflectance spectra of blood cultures. This new model is based on the photon diffusion theory and Mie scattering theory that have been formulated to account for multiple scattering populations and absorptive components. This study stresses the significance of the thorough solution of the scattering and absorption problem in order to accurately resolve for optically relevant parameters of blood culture components. With advantages of being calibration-free and computationally fast, the new model has two basic requirements. First, wavelength-dependent refractive indices of the basic chemical constituents of blood culture components are needed. Second, multi-wavelength measurements or at least the measurements of characteristic wavelengths equal to the degrees of freedom, i.e. number of optically relevant parameters, of blood culture system are required. The blood culture analysis model was tested with a large number of diffuse reflectance spectra of blood culture samples characterized by an extensive range of the relevant parameters.

© 2008 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(300.0300) Spectroscopy : Spectroscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 4, 2008
Revised Manuscript: October 16, 2008
Manuscript Accepted: October 21, 2008
Published: October 22, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Yulia M. Serebrennikova, Jennifer M. Smith, Debra E. Huffman, German F. Leparc, and Luis H. García-Rubio, "Quantitative interpretations of Visible-NIR reflectance spectra of blood," Opt. Express 16, 18215-18229 (2008)

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