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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 23 — Nov. 10, 2008
  • pp: 18714–18724

Localization of a fluorescent source without numerical fitting

Sean B. Andersson  »View Author Affiliations


Optics Express, Vol. 16, Issue 23, pp. 18714-18724 (2008)
http://dx.doi.org/10.1364/OE.16.018714


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Abstract

We present an algebraic solution to the problem of localizing a single fluorescent particle with sub-diffraction-limit accuracy. The algorithm is derived and its performance studied experimentally. Isolated 20 nm fluorescent beads were imaged using a wide-field microscope at two different positions separated by 100 nm and at a range of signal-to-noise ratios (SNR). The data were analyzed using both the new algorithm and the standard approach of fitting the data to a Gaussian profile. Results indicate that the proposed approach is nearly as accurate as Gaussian fitting across a wide range of SNR while executing over 200 times faster. In addition, the new algorithm is able to localize at lower SNR than the fitting method.

© 2008 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(180.2520) Microscopy : Fluorescence microscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Microscopy

History
Original Manuscript: August 29, 2008
Revised Manuscript: October 9, 2008
Manuscript Accepted: October 24, 2008
Published: October 29, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Sean Andersson, "Localization of a fluorescent source without numerical fitting," Opt. Express 16, 18714-18724 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-23-18714


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References

  1. M. Lakadamyali, M. J. Rust, H. P. Babcock, and X. Zhuang, "Visualizing infection of individual influenza viruses," P. Natl. Acad. Sci USA 100, 9280-9285 (2003). [CrossRef]
  2. C. Kural, H. Balci, and P. R. Selvin, "Molecular motors one at a time: FIONA to the rescue," J. Phys. Condens. Matt. 27, S3979-S3995 (2005). [CrossRef]
  3. C. K. Payne, "Imaging gene delivery with fluorescence microscopy," Nanomedicine 2, 847-860 (2007). [CrossRef] [PubMed]
  4. W. E. Moerner, "New directions in single-molecule imaging and analysis," P. Natl. Acad. Sci USA 104, 596-602 (2007). [CrossRef]
  5. C. Joo, H. Balci, Y. Ishitsuka, C. Buranachai, and T. Ha, "Advances in single-molecule fluorescence methods for molecular biology," Annu. Rev. Biochem. 77, 51-76 (2008). [CrossRef] [PubMed]
  6. M. K. Cheezum, W. F. Walker, and W. H. Guilford, "Quantitative comparison of algorithms for tracking single fluorescent particles," Biophys. J. 81, 2378-2388 (2001). [CrossRef] [PubMed]
  7. A. J. Berglund, M. D. McMahon, J. J. McClelland, and J. A. Liddle, "Fast, bias-free algorithm for tracking single particles with variable size and shape," Opt. Express 16, 064-075 (2008). [CrossRef]
  8. R. E. Thompson, D. R. Larson, and W. W. Webb, "Precise nanometer localization analysis for individual fluorescent probes," Biophys. J. 82, 2775-2783 (2002). [CrossRef] [PubMed]
  9. R. J. Ober, S. Ram, and E. S. Ward, "Localization accuracy in single-molecule microscopy," Biophys. J. 86, 1185-1200 (2007). [CrossRef]
  10. S. Ram, E. S. Ward, and R. J. Ober, "A stochastic analysis of performance limits for optical microscopes," Multdim. Syst. Sign. P. 17, 27-57 (2006). [CrossRef]
  11. A. J. Berglund and H. Mabuchi, "Tracking-FCS: Fluorescence correlation spectroscopy of individual particles," Opt. Express 13, 8069-8082 (2005). [CrossRef] [PubMed]
  12. S. B. Andersson, "Tracking a single fluorescent molecule with a confocal microscope," Appl. Phys. B 80, 809-816 (2005). [CrossRef]
  13. V. Levi, Q. Ruan, and E. Gratton, "3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells," Biophys. J. 88, 2919-2928 (2005). [CrossRef] [PubMed]
  14. S. Bancroft, "An algebraic solution of the GPS pseudorange equations," IEEE T. Aero. Elec. Sys. AES-21, 56-59 (1985). [CrossRef]
  15. T. Sun and S. B. Andersson, "Precise 3-D localization of fluorescent probes without numerical fitting," in Proceedings of the International Conference of IEEE Engineering in Medicine and Biology Society (IEEE, 2007) pp. 4181-4184. [PubMed]
  16. S. B. Andersson, "Precise localization of fluorescent probes without numerical fitting," in Proceedings of IEEE International Symposium on Biomedical Imaging (ISBI) (IEEE, 2007), pp. 252-255.
  17. S. B. Andersson, "Position estimation of fluorescent probes in a confocal microscope," in Proceedings of IEEE Conference on Decision and Control (IEEE, 2007), pp. 4950-4955.
  18. R. Juskaitis, "Measuring the real point spread function of high numerical aperture microscope objective lenses," in Handbook of Biological Confocal Microscopy, J. B. Pawley, ed. (Springer, 2006), pp. 239-250. [CrossRef]
  19. A. Ben-Israel and T. N. E. Greville, Generalized Inverses: Theory and Applications, Pure and Applied Mathematics (John Wiley and Sons, 1974).
  20. A. Papoulis, Probability, Random Variables, and Stochastic Processes (McGraw-Hill, 1991).

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