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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 23 — Nov. 10, 2008
  • pp: 18764–18769

Focal modulation microscopy

Nanguang Chen, Chee-Howe Wong, and Colin J. R. Sheppard  »View Author Affiliations

Optics Express, Vol. 16, Issue 23, pp. 18764-18769 (2008)

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We report a novel light microscopy method for high resolution molecular imaging of thick biological tissues with one photon excited fluorescence. Effective optical sectioning and diffraction limited spatial resolution are achieved when imaging deep inside a multiple-scattering medium by the use of focal modulation, a technique for suppressing the background fluorescence signal excited by scattered light. Our method has been validated with animal tissue and an imaging depth around 600 microns has been demonstrated.

© 2008 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.2520) Microscopy : Fluorescence microscopy
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:

Original Manuscript: September 22, 2008
Revised Manuscript: October 19, 2008
Manuscript Accepted: October 19, 2008
Published: October 29, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Nanguang Chen, Chee-Howe Wong, and Colin J. Sheppard, "Focal modulation microscopy," Opt. Express 16, 18764-18769 (2008)

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