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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 24 — Nov. 24, 2008
  • pp: 19462–19479

Partially coherent image formation in differential interference contrast (DIC) microscope

Shalin B. Mehta and Colin J. R. Sheppard  »View Author Affiliations


Optics Express, Vol. 16, Issue 24, pp. 19462-19479 (2008)
http://dx.doi.org/10.1364/OE.16.019462


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Abstract

Some different image formation models have been proposed for Nomarski’s differential interference contrast (DIC) microscope. However, the nature of coherence of illumination in DIC, of key importance in image formation, remains to be elucidated. We present a partially coherent image formation model for DIC and demonstrate that DIC microscope images the coherent difference of shifted replicas of the specimen; but imaging of the each component is partially coherent. Partially coherent transfer functions are presented for various DIC configurations. Plots of these transfer functions and experimental images provide quantitative comparison among various DIC configurations and elucidate their imaging properties. Approximations for weak or slowly varying specimens are also given. These improved models should be of great value in designing phase retrieval algorithms for DIC.

© 2008 Optical Society of America

OCIS Codes
(030.1670) Coherence and statistical optics : Coherent optical effects
(050.1960) Diffraction and gratings : Diffraction theory
(110.2990) Imaging systems : Image formation theory
(180.3170) Microscopy : Interference microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 2, 2008
Revised Manuscript: October 13, 2008
Manuscript Accepted: October 13, 2008
Published: November 10, 2008

Virtual Issues
Vol. 4, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Shalin B. Mehta and Colin J. Sheppard, "Partially coherent image formation in differential interference contrast (DIC) microscope," Opt. Express 16, 19462-19479 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-24-19462


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