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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 25 — Dec. 8, 2008
  • pp: 20258–20265

Simultaneous widefield single molecule orientation and FRET microscopy in cells

S. E. D. Webb, D. J. Rolfe, S. R. Needham, S. K. Roberts, D. T. Clarke, C. I. McLachlan, M. P. Hobson, and M. L. Martin-Fernandez  »View Author Affiliations

Optics Express, Vol. 16, Issue 25, pp. 20258-20265 (2008)

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We combine single molecule fluorescence orientation imaging with single-pair fluorescence resonance energy transfer microscopy, using a total internal reflection microscope. We show how angles and FRET efficiencies can be determined for membrane proteins at the single molecule level and provide data from the epidermal growth factor receptor system in cells.

© 2008 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 10, 2008
Revised Manuscript: November 12, 2008
Manuscript Accepted: November 12, 2008
Published: November 24, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

S. E. D. Webb, D. J. Rolfe, S. R. Needham, S. K. Roberts, D. T. Clarke, C. I. McLachlan, M. P. Hobson, and M. L. Martin-Fernandez, "Simultaneous widefield single molecule orientation and FRET microscopy in cells," Opt. Express 16, 20258-20265 (2008)

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