OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 25 — Dec. 8, 2008
  • pp: 20734–20741

Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams

K.J. Moh, X.-C. Yuan, J. Bu, S. W. Zhu, and Bruce Z. Gao  »View Author Affiliations

Optics Express, Vol. 16, Issue 25, pp. 20734-20741 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (897 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at the cell-substrate interface of a biological cell in aqueous media. The technique is based on the optical excitation by focused radially polarized beams of localized surface plasmons, which forms a virtual probe on the metal substrate. The intensity distribution at the back focal plane of the objective lens enables quantitative measurements to be made of the cell-substrate contact. The acquired data is then visualized in the form of a local refractive index map.

© 2008 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(180.5810) Microscopy : Scanning microscopy
(240.6680) Optics at surfaces : Surface plasmons
(260.5430) Physical optics : Polarization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 3, 2008
Revised Manuscript: November 21, 2008
Manuscript Accepted: November 23, 2008
Published: December 1, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

K. J. Moh, X.-C. Yuan, J. Bu, S. W. Zhu, and Bruce Z. Gao, "Surface plasmon resonance imaging of cell-substrate contacts with radially polarized beams," Opt. Express 16, 20734-20741 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J. Homola, S. S. Yee, and G. Gauglitz, "Surface plasmon resonance sensors: review," Sens. Actuators B 54,3-15 (1999). [CrossRef]
  2. J. M. Brockman, B. P. Nelson, and R. M. Corn, "Surface Plasmon Resonance Imaging Measurements of Ultrathin Organic films," Annu. Rev. Phys. Chem. 51, 41-63 (2000). [CrossRef] [PubMed]
  3. H. Kano, S. Mizuguchi, and S. Kawata, "Excitation of surface plasmon polaritons by a focused laser beam," J. Opt. Soc. Am. B 15, 1381-1386 (1998). [CrossRef]
  4. H. Kano and W. Knoll, "Locally excited surface-plasmon-polaritons for thickness measurement of LBK films," Optic. Commun. 153, 235-239 (1998). [CrossRef]
  5. H. Kano and W. Knoll, "A scanning microscope employing localized surface-plasmon-polaritons as a sensing probe," Optic. Commun. 182, 11-15 (2000). [CrossRef]
  6. K. Watanabe, N. Horiguchi, and H. Kano, "Optimized measurement probe of the localized surface plasmon microscope by using radially polarized illumination," Appl. Opt. 46, 4985-4990 (2007). [CrossRef] [PubMed]
  7. M. G. Somekh, S. G. Liu, T. S. Velinov, and C. W. See, "Optical V(z) for high-resolution 2Pi surface plasmon microscopy," Opt. Lett. 25, 823-825 (2000). [CrossRef]
  8. M. G. Somekh, S. G. Liu, T. S. Velinov, and C. W. See, "High-Resolution Scanning Surface-Plasmon Microscopy," Appl. Opt. 39, 6279-6287 (2000). [CrossRef]
  9. Q. Zhan, "Evanescent Bessel beam generation via surface plasmon resonance excitation by a radially polarized beam," Optic. Lett. 31, 1726-1728 (2006). [CrossRef]
  10. D. Axelrod, "Cell-Substrate Contacts Illuminated by Total Internal Reflection Fluorescence," J. Cell Biol. 89, 141-145 (1981). [CrossRef] [PubMed]
  11. K.-F. Giebel, C. Bechinger, S. Herminghaus, M. Riedel, P. Leiderer, U. Weiland, and M. Bastmeyer, "Imaging of Cell/substrate contacts of living cells with surface Plasmon resonance microscopy," Biophys. J. 76, 509-516 (1999). [CrossRef] [PubMed]
  12. M. Mahadi Abdul Jamil, M. C. T. Denyer, M. Youseffi, S. T. Britland, S. Liu, C. W. See, M. G. Somekh, and J. Zhang, "Imaging of the cell surface interface using objective coupled widefield surface plasmon microscopy," J. Struct. Biol. 164, 75-80 (2008). [CrossRef] [PubMed]
  13. E. Descrovi, V. Paeder, L. Vaccaro, and H. Herzig "A virtual optical probe based on localized Surface Plasmon Polaritons," Opt. Express 13, 7017-7027 (2005). [CrossRef] [PubMed]
  14. T. Grosjean, D. Courjon, and D. Van Labeke, "Bessel beams as virtual tips for near-field optics, " J. Microsc. 210, 319-323 (2003). [CrossRef] [PubMed]
  15. K. J. Moh, X. C. Yuan, J. Bu, D. K. Y. Low, and R. E. Burge, "Direct noninterference cylindrical vector beam generation applied in the femtosecond regime," Appl. Phys. Lett. 89, 251114 (2006). [CrossRef]
  16. K. J. Moh, X. C. Yuan, J. Bu, R. E. Burge, and B. Z. Gao, "Generating radial or azimuthal polarization by axial sampling of circularly polarized vortex beams," Appl. Opt. 46, 7544-7551 (2007). [CrossRef] [PubMed]
  17. R. Dorn, S. Quabis, and G. Leuchs, "Sharper Focus for a Radially Polarized Light Beam," Physical Rev. Lett. 91, 233901-233901 (2003). [CrossRef]
  18. A. Bouhelier, J. Renger, M. R. Beversluis, and L. Novotny, "Plasmon-coupled tip-enhanced near-field optical microscopy," J. Microsc. 210, 220-224 (2003). [CrossRef] [PubMed]
  19. D. P. Biss, K. S. Youngworth, and T. G. Brown, "Dark-field imaging with cylindrical-vector beams," Appl. Opt. 45, 470-479 (2006). [CrossRef] [PubMed]
  20. J. A. Stone and J. H. Zimmerman, "Engineering Metrology Toolbox, Index of Refraction of Air" (NIST, 2000), http://emtoolbox.nist.gov/Wavelength/Edlen.asp.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited