OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 26 — Dec. 22, 2008
  • pp: 21843–21848

Real-time extended depth of field microscopy

Edward J. Botcherby, Martin J. Booth, Rimas Juškaitis, and Tony Wilson  »View Author Affiliations

Optics Express, Vol. 16, Issue 26, pp. 21843-21848 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (974 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We describe an optical microscope system whose focal setting can be changed quickly without moving the objective lens or specimen. Using this system, diffraction limited images can be acquired from a wide range of focal settings without introducing optical aberrations that degrade image quality. We combine this system with a real time Nipkow disc based confocal microscope so as to permit the acquisition of extended depth of field images directly in a single frame of the CCD camera. We also demonstrate a simple modification that enables extended depth of field images to be acquired from different angles of perspective, where the angle can be changed over a continuous range by the user in real-time.

© 2008 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: September 8, 2008
Revised Manuscript: November 20, 2008
Manuscript Accepted: November 24, 2008
Published: December 17, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

Edward J. Botcherby, Martin J. Booth, Rimas Juskaitis, and Tony Wilson, "Real-time extended depth of field microscopy," Opt. Express 16, 21843-21848 (2008)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic Press, London, 1984).
  2. M. A. A. Neil, R. Ju¡skaitis, T. Wilson, Z. J. Laczik, and V. Sarafis, "Optimized pupil-plane filters for confocal microscope point-spread function engineering," Opt. Lett. 25,245-247 (2000). [CrossRef]
  3. E. Botcherby, R. Ju¡skaitis, and T. Wilson, "Scanning two photon fluorescence microscopy with extended depth of field," Opt. Comm. 268,253-260 (2006). [CrossRef]
  4. N. George and W. Chi, "Extended depth of field using a logarithmic asphere," J. Opt. A: Pure Appl. Opt. 5,S157-S163 (2003). [CrossRef]
  5. E. R. Dowski and W. T. Cathey, "Extended depth of field through wave-front coding," Appl. Opt. 34,1859-1866 (1995). [CrossRef] [PubMed]
  6. M. Born and E. Wolf, Principles of Optics (Pergamon Press, 6th edition, 1983).
  7. E. Botcherby, R. Ju¡skaitis, M. Booth, and T. Wilson, "Aberration-free optical refocusing in high numerical aperture microscopy," Opt. Lett. 32,2007-2009 (2007). [CrossRef] [PubMed]
  8. E. Botcherby, R. Ju¡skaitis, M. Booth, and T. Wilson, "An optical technique for remote focusing in microscopy," Opt. Comm. 281,880-887 (2008). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

Supplementary Material

» Media 1: MOV (136 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited