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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 26 — Dec. 22, 2008
  • pp: 22083–22089

Interference terahertz label-free imaging for protein detection on a membrane

Yuichi Ogawa, Shin’ichiro Hayashi, Masato Oikawa, Chiko Otani, and Kodo Kawase  »View Author Affiliations

Optics Express, Vol. 16, Issue 26, pp. 22083-22089 (2008)

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We demonstrate a highly sensitive imaging method combined a terahertz time-domain spectroscopy and an interference effect for label-free protein detection on a polyvinylidene difluoride membrane. The method is based on terahertz time-domain spectroscopy and uses an interference effect. Biotin is linked to the membrane using poly ethylene glycol or poly ethylene glycol methyl ether to prevent it from being washed off. Binding of the biotin with streptavidin is then observed by measuring the terahertz signal change due to the variation of the membrane refractive index. We demonstrate the detection of the binding streptavidin protein in gradually decreasing concentrations, down to 27 ng mm-2, using the image recorded at 1.5 THz.

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(110.6795) Imaging systems : Terahertz imaging
(170.6795) Medical optics and biotechnology : Terahertz imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 20, 2008
Revised Manuscript: November 22, 2008
Manuscript Accepted: November 26, 2008
Published: December 19, 2008

Virtual Issues
Vol. 4, Iss. 2 Virtual Journal for Biomedical Optics

Yuichi Ogawa, Shin'ichiro Hayashi, Masato Oikawa, Chiko Otani, and Kodo Kawase, "Interference terahertz label-free imaging for protein detection on a membrane," Opt. Express 16, 22083-22089 (2008)

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