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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 3 — Feb. 4, 2008
  • pp: 2153–2167

Polarization microscopy with stellated gold nanoparticles for robust, in-situ monitoring of biomolecules

Jesse Aaron, Elder de la Rosa, Kort Travis, Nathan Harrison, Justin Burt, Miguel José-Yacamán, and Konstantin Sokolov  »View Author Affiliations

Optics Express, Vol. 16, Issue 3, pp. 2153-2167 (2008)

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Advances in plasmonic nanoparticle synthesis afford new opportunities for biosensing applications. Here, we apply a combination of a new type of plasmonic nanomaterial – stellated nanoparticles, and polarization-sensitive darkfield microscopy for detecting molecular assemblies and tracking of individual epidermal growth factor receptors within single live cells with high signal-to-background ratio. Depolarization of linear polarized light by stellated nanoparticles is over 15-fold more efficient than similarly-sized spheroidal nanoparticles. This efficient light depolarization allows robust detection of molecules labeled with stellated nanoparticles in cross-polarized imaging where the intrinsic light scattering from cells is significantly reduced. The imaging can be carried out with single molecule sensitivity for essentially unlimited time with no signal degradation.

© 2008 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(260.5430) Physical optics : Polarization
(250.5403) Optoelectronics : Plasmonics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 26, 2007
Revised Manuscript: January 17, 2008
Manuscript Accepted: January 20, 2008
Published: January 31, 2008

Virtual Issues
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics

Jesse Aaron, Elder de la Rosa, Kort Travis, Nathan Harrison, Justin Burt, Miguel José-Yacamán, and Konstantin Sokolov, "Polarization microscopy with stellated gold nanoparticles for robust monitoring of molecular assemblies and single biomolecules," Opt. Express 16, 2153-2167 (2008)

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