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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 3 — Feb. 4, 2008
  • pp: 2168–2175

Simultaneous forward and epi-CARS microscopy with a single detector by timecorrelated single photon counting

Iwan W. Schie, Tyler Weeks, Gregory P. McNerney, Samantha Fore, Juliana K. Sampson, Sebastian Wachsmann-Hogiu, John C. Rutledge, and Thomas Huser  »View Author Affiliations


Optics Express, Vol. 16, Issue 3, pp. 2168-2175 (2008)
http://dx.doi.org/10.1364/OE.16.002168


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Abstract

We present a novel scheme to simultaneously detect coherent anti-Stokes Raman scattering (CARS) microscopy signals in the forward (F) and backward (epi - E) direction with a single avalanche photodiode (APD) detector using time-correlated single photon counting (TCSPC). By installing a mirror at a well-defined distance above the sample the forward-scattered F-CARS signal is reflected back into the microscope objective leading to spatial overlap of the F and E-CARS signals. Due to traveling an additional distance the F-CARS signal is time delayed relative to the E-CARS signal. TCSPC then allows for the two signals to be resolved in the time domain. This results in an efficient, simple, and compact method of CARS signal detection. We demonstrate this technique by analyzing forward and backward CARS signals obtained by imaging living adipocyte cells derived from human mesenchymal stem cells.

© 2008 Optical Society of America

OCIS Codes
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(190.5650) Nonlinear optics : Raman effect
(220.4830) Optical design and fabrication : Systems design
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: December 10, 2007
Revised Manuscript: January 24, 2008
Manuscript Accepted: January 27, 2008
Published: January 31, 2008

Virtual Issues
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Iwan W. Schie, Tyler Weeks, Gregory P. McNerney, Samantha Fore, Juliana K. Sampson, Sebastian Wachsmann-Hogiu, John C. Rutledge, and Thomas Huser, "Simultaneous forward and epi-CARS microscopy with a single detector by timecorrelated single photon counting," Opt. Express 16, 2168-2175 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-3-2168


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