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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 4 — Feb. 18, 2008
  • pp: 2699–2708

Selective imaging of saturated and unsaturated lipids by wide-field CARS-microscopy

Christoph Heinrich, Alexander Hofer, Andreas Ritsch, Christian Ciardi, Stefan Bernet, and Monika Ritsch-Marte  »View Author Affiliations


Optics Express, Vol. 16, Issue 4, pp. 2699-2708 (2008)
http://dx.doi.org/10.1364/OE.16.002699


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Abstract

Wide-field Coherent Anti-Stokes Raman Scattering (CARS) microscopy is employed to identify saturated and unsaturated fatty acids in micro-emulsions and cells, using the ratio between the strong -C-H CARS signal at 2850cm-1 and the weak signal of the =C-H vibration around 3015cm-1 for distinction. Quantitative CARS imaging at the =C-H resonance is challenging, since it yields only a low CARS signal, and small differences on the order of 5% in the concentration of polyunsaturated fatty lipids have to be detected. For this purpose we draw advantage of the high signal-to-noise ratio of wide-field CARS microscopy that is achieved by an excitation geometry involving a “sheet-of-light”-type illumination.

© 2008 Optical Society of America

OCIS Codes
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Spectroscopy

History
Original Manuscript: November 14, 2007
Revised Manuscript: January 23, 2008
Manuscript Accepted: January 27, 2008
Published: February 12, 2008

Virtual Issues
Vol. 3, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Christoph Heinrich, Alexander Hofer, Andreas Ritsch, Christian Ciardi, Stefan Bernet, and Monika Ritsch-Marte, "Selective imaging of saturated and unsaturated lipids by wide-field CARS-microscopy," Opt. Express 16, 2699-2708 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-4-2699


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  25. Altogether, 20 liposomes in 5 cells fed on stearic acid, and 28 liposomes in 7 cells fed on arachidonic acid were measured. The measured content of double bindings in the cells fed on stearic acid was constant within a relative variation of ±10%, whereas there was a strong fluctuation of ±40% within the group fed on arachidonic acid. On the other hand, both groups showed only a negligible concentration difference between the individual liposomes within single cells.

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