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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 6 — Mar. 17, 2008
  • pp: 3496–3503

Design of a system to measure light scattering from individual cells excited by an acoustic wave

Ramona Georgescu, Damir Khismatullin, R. Glynn Holt, Jean Luc Castagner, Ousama A’amar, and Irving J. Bigio  »View Author Affiliations


Optics Express, Vol. 16, Issue 6, pp. 3496-3503 (2008)
http://dx.doi.org/10.1364/OE.16.003496


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Abstract

A system to measure light scattering from individual cells excited by an acoustic wave was designed, and tests were performed on live Jurkat cells. Cells passing in a laminar stream within a water bath were excited by a focused ultrasound pulse, while the scattered light from a laser beam was monitored at various scattering angles. The cells were modeled as viscoelastic liquid drops, which return to equilibrium via shape oscillations after an acoustically-induced deformation. The Fast Fourier Transform of the scattered light signal was used to extract information about the highly-damped resonant frequencies of the cells, and the detected frequencies are consistent with theoretical predictions.

© 2008 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(290.4020) Scattering : Mie theory
(170.1065) Medical optics and biotechnology : Acousto-optics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 14, 2007
Revised Manuscript: February 4, 2008
Manuscript Accepted: February 6, 2008
Published: March 3, 2008

Virtual Issues
Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Ramona Georgescu, Damir Khismatullin, R. Glynn Holt, Jean Luc Castagner, Ousama A’amar, and Irving J. Bigio, "Design of a system to measure light scattering from individual cells excited by an acoustic wave," Opt. Express 16, 3496-3503 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-6-3496


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