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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 6 — Mar. 17, 2008
  • pp: 4237–4249

Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat brain tissue reveals spatially distinct NADH binding

Thomas H. Chia, Anne Williamson, Dennis D. Spencer, and Michael J. Levene  »View Author Affiliations

Optics Express, Vol. 16, Issue 6, pp. 4237-4249 (2008)

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Two-photon fluorescence lifetime imaging (FLIM) of molecules can reveal important information on the local microenvironment. NADH, an intrinsic fluorescent molecule and ubiquitous metabolic co-enzyme, has a lifetime that depends strongly on enzymatic binding. We present a custom image-processing algorithm for raw fluorescence lifetime and amplitude data that produces an image showing spatially distinct NADH fluorescence lifetimes in slices of rat and human brain. NADH FLIM images were collected in control and epileptic rat tissue. Differences in spatial patterns of NADH lifetimes support the hypothesis that NADH binding, and thus metabolic capacity, is significantly different between groups. This type of analysis can provide information on metabolic states in pathological material.

© 2008 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.5810) Medical optics and biotechnology : Scanning microscopy
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.2520) Microscopy : Fluorescence microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 22, 2008
Revised Manuscript: March 5, 2008
Manuscript Accepted: March 11, 2008
Published: March 13, 2008

Virtual Issues
Vol. 3, Iss. 4 Virtual Journal for Biomedical Optics

Thomas H. Chia, Anne Williamson, Dennis D. Spencer, and Michael J. Levene, "Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat brain tissue reveals spatially distinct NADH binding," Opt. Express 16, 4237-4249 (2008)

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