Imaging polyhedral inclusion bodies of nuclear polyhedrosis viruses with second harmonic generation microscopy
Optics Express, Vol. 16, Issue 8, pp. 5602-5608 (2008)
http://dx.doi.org/10.1364/OE.16.005602
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Abstract
We studied the polarization anisotropy of second harmonic generation (SHG) in polyhedral inclusion bodies (PIBs) of nuclear polyhedrosis viruses (NPV). Due to a body-centered-cubic arrangement of polyhedrin trimers, a characteristic SHG polarization property with a mixture of I23 and I3 symmetry was measured from PIBs. With this characteristic SHG anisotropy, it provides an intrinsic nonlinear signal for virus infection studies in living cells. With multimodal harmonic generation microscopy, we also demonstrated 3D imaging on PIBs of NPV in living cells. The distribution and the number of PIBs in intact infected cells can be revealed without the help of fluorescent labeling.
© 2008 Optical Society of America
OCIS Codes
(190.3970) Nonlinear optics : Microparticle nonlinear optics
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Microscopy
History
Original Manuscript: January 15, 2008
Revised Manuscript: March 14, 2008
Manuscript Accepted: March 25, 2008
Published: April 7, 2008
Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Tzu-Ming Liu, Yen-Wei Lee, Chieh-Feng Chang, Shih-Chia Yeh, Chung-Hsiung Wang, Shi-Wei Chu, and Chi-Kuang Sun, "Imaging polyhedral inclusion bodies of nuclear polyhedrosis viruses with second harmonic generation microscopy," Opt. Express 16, 5602-5608 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-8-5602
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