OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 8 — Apr. 14, 2008
  • pp: 5602–5608

Imaging polyhedral inclusion bodies of nuclear polyhedrosis viruses with second harmonic generation microscopy

Tzu-Ming Liu, Yen-Wei Lee, Chieh-Feng Chang, Shih-Chia Yeh, Chung-Hsiung Wang, Shi-Wei Chu, and Chi-Kuang Sun  »View Author Affiliations

Optics Express, Vol. 16, Issue 8, pp. 5602-5608 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (160 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We studied the polarization anisotropy of second harmonic generation (SHG) in polyhedral inclusion bodies (PIBs) of nuclear polyhedrosis viruses (NPV). Due to a body-centered-cubic arrangement of polyhedrin trimers, a characteristic SHG polarization property with a mixture of I23 and I3 symmetry was measured from PIBs. With this characteristic SHG anisotropy, it provides an intrinsic nonlinear signal for virus infection studies in living cells. With multimodal harmonic generation microscopy, we also demonstrated 3D imaging on PIBs of NPV in living cells. The distribution and the number of PIBs in intact infected cells can be revealed without the help of fluorescent labeling.

© 2008 Optical Society of America

OCIS Codes
(190.3970) Nonlinear optics : Microparticle nonlinear optics
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: January 15, 2008
Revised Manuscript: March 14, 2008
Manuscript Accepted: March 25, 2008
Published: April 7, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Tzu-Ming Liu, Yen-Wei Lee, Chieh-Feng Chang, Shih-Chia Yeh, Chung-Hsiung Wang, Shi-Wei Chu, and Chi-Kuang Sun, "Imaging polyhedral inclusion bodies of nuclear polyhedrosis viruses with second harmonic generation microscopy," Opt. Express 16, 5602-5608 (2008)

Sort:  Year  |  Journal  |  Reset  


  1. J. H. Chang, J. Y. Choi, B. R. Jin, J. Y. Roh, J. A. Olszewski, S. J. Seo, D. R. O'Reilly, and Y. H. Je, "An improved baculovirus insecticide producing occlusion bodies that contain Bacillus thuringiensis insect toxin," J. Invertebr. Pathol. 84, 30-37 (2003). [CrossRef] [PubMed]
  2. P. J. Harshman, T. K. Gustafson, and P. Kelley, "Baculovirus-mediated gene transfer into mammalian cells," Proc. Natl. Acad. Sci. U.S.A. 93, 2348-2352 (1996). [CrossRef]
  3. T.-C. Lee, S.-M. Yu, and Y. C. Chao. "Fusion of foreign protein genes to the occlusion body gene of the baculovirus to assist protein isolation and antibody production," J. Genet. Mol. Biol. 16, 138-150 (2005).
  4. C.-K. Sun, S.-W. Chu, S.-Y. Chen, T.-H. Tsai, T.-M. Liu, C.-Y. Lin, and H.-J. Tsai, "Higher harmonic generation microscopy for developmental biology," J. Struct. Biol. 147, 19- 30 (2004). [CrossRef] [PubMed]
  5. Asian Vegetable Research and Development Center (AVRDC). AVRDC Annual Report 2004, W. Easdown and T. Kalb, eds., (AVRDC, Shanhua, Tainan, Taiwan, 2007).
  6. S. C. Yeh, S. T. Lee, C Y. Wu, and C. H. Wang. "A cell line (NTU-MV) established from Maruca vitrata (Lepidoptera: pyralidae): characterization, viral susceptibility, and polyhdra production," J. Invertebr. Pathol. 96, 138-146 (2007). [CrossRef] [PubMed]
  7. S.-Y. Chen, C.-S. Hsieh, S.-W. Chu, C.-Y. Lin, C.-Y. Ko, Y.-C. Chen, H.-J. Tsai, C.-H. Hu, and C.-K. Sun, "Noninvasive harmonics optical microscopy for long-term observation of embryonic nervous system development in vivo," J. Biomed. Opt. 11, 054022 (2006). [CrossRef] [PubMed]
  8. R. M. Williams, W. R. Zipfel, and W. W. Webb, "Interpreting second-harmonic generation images of collagen I fibrils," Biophys. J. 88, 1377-1386 (2005). [CrossRef]
  9. J.-X. Cheng and X. S. Xie, "Green’s function formulation for third-harmonic generation microscopy," J. Opt. Soc. Am. B 19, 1604-1610 (2002). [CrossRef]
  10. K. Anduleit, G. Sutton, J. M. Diprose, P. P. C. Mertens, J. M. Grimes, and D. I. Stuart, "Crystal lattice as biological phenotype for insect viruses," Protein Sci. 14, 2741-2743 (2005). [CrossRef] [PubMed]
  11. F. Coulibaly, E. Chiu, K. Ikeda, S. Gutmann, P. W. Haebel, C. Schulze-Briese, H. Mori, and P. Metcalf, "The molecular organization of cypovirus polyhedra," Nature 446, 97-101 (2007). [CrossRef] [PubMed]
  12. A. Yariv, Optical Electronics in Modern Communications, 5th ed. (Oxford University Press, 1997), pp. 330.
  13. S.-W. Chu, S.-P. Tai, C.-K. Sun, and C.-H. Lin, "Selective imaging in second-harmonic-generation microscopy by polarization manipulation," Appl. Phys. Lett. 91, 103903 (2007). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited