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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 8 — Apr. 14, 2008
  • pp: 5782–5789

Microfluidic CARS cytometry

Han-Wei Wang, Ning Bao, Thuc T. Le, Chang Lu, and Ji-Xin Cheng  »View Author Affiliations


Optics Express, Vol. 16, Issue 8, pp. 5782-5789 (2008)
http://dx.doi.org/10.1364/OE.16.005782


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Abstract

Coherent anti-stokes Raman scattering (CARS) flow cytometry was demonstrated by combining a laser-scanning CARS microscope with a polydimethylsiloxane (PDMS) based microfluidic device. Line-scanning across the hydrodynamically focused core stream was performed for detection of flowing objects. Parameters were optimized by utilizing polystyrene beads as flowing particles. Population measurements of adipocytes isolated from mouse fat tissues demonstrated the viability of microfluidic CARS cytometry for quantitation of adipocyte size distribution. CARS cytometry could be a new modality for quantitative analysis with vibrational selectivity.

© 2008 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 8, 2008
Revised Manuscript: April 4, 2008
Manuscript Accepted: April 8, 2008
Published: April 10, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Han-Wei Wang, Ning Bao, Thuc L. Le, Cheng Lu, and Ji-Xin Cheng, "Microfluidic CARS cytometry," Opt. Express 16, 5782-5789 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-8-5782


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