OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 8 — Apr. 14, 2008
  • pp: 5782–5789

Microfluidic CARS cytometry

Han-Wei Wang, Ning Bao, Thuc T. Le, Chang Lu, and Ji-Xin Cheng  »View Author Affiliations

Optics Express, Vol. 16, Issue 8, pp. 5782-5789 (2008)

View Full Text Article

Enhanced HTML    Acrobat PDF (334 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Coherent anti-stokes Raman scattering (CARS) flow cytometry was demonstrated by combining a laser-scanning CARS microscope with a polydimethylsiloxane (PDMS) based microfluidic device. Line-scanning across the hydrodynamically focused core stream was performed for detection of flowing objects. Parameters were optimized by utilizing polystyrene beads as flowing particles. Population measurements of adipocytes isolated from mouse fat tissues demonstrated the viability of microfluidic CARS cytometry for quantitation of adipocyte size distribution. CARS cytometry could be a new modality for quantitative analysis with vibrational selectivity.

© 2008 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 8, 2008
Revised Manuscript: April 4, 2008
Manuscript Accepted: April 8, 2008
Published: April 10, 2008

Virtual Issues
Vol. 3, Iss. 5 Virtual Journal for Biomedical Optics

Han-Wei Wang, Ning Bao, Thuc L. Le, Cheng Lu, and Ji-Xin Cheng, "Microfluidic CARS cytometry," Opt. Express 16, 5782-5789 (2008)

Sort:  Year  |  Journal  |  Reset  


  1. H. M. Shapiro, Practical Flow Cytometry, 4th ed. (Wiley Liss, New York, 2003). [CrossRef]
  2. C. D. Jennings and K. A. Foon, "Recent Advances in Flow Cytometry: Application to the Diagnosis of Hematologic Malignancy," Blood 90, 2863-2892 (1997). [PubMed]
  3. T. D. Chung and H. C. Kim, "Recent advances in miniaturized microfluidic flow cytometry for clinical use," Electrophoresis 28, 4511-4520 (2007). [CrossRef] [PubMed]
  4. A. Gordon, A. Colman-Lerner, T. E. Chin, K. R. Benjamin, R. C. Yu, and R. Brent, "Single-cell quantification of molecules and rates using open-source microscope-based cytometry," Nat. Methods 4, 175-181 (2007). [CrossRef] [PubMed]
  5. S. P. Perfetto, P. K. Chattopadhyay, and M. Roederer, "Seventeen-colour flow cytometry: unravelling the immune system," Nat. Rev. Immun. 4, 648-655 (2004). [CrossRef]
  6. J. X. Cheng, "Coherent anti-stokes Raman scattering microscopy," Appl. Spectrosc. 61, 197A-208A (2007). [CrossRef]
  7. M. Muller and A. Zumbusch, "Coherent anti-Stokes Raman Scattering Microscopy," Chem. PhysChem. 8, 2156-2170 (2007). [CrossRef]
  8. J. X. Cheng and X. S. Xie, "Coherent anti-Stokes Raman scattering microscopy: instrumentation, theory, and applications," J. Phys. Chem. B 108, 827-840 (2004). [CrossRef]
  9. H.-Y. Wang and C. Lu, "Microfluidic chemical cytometry based on modulation of local field strength," Chem. Commun. 3528-3530 (2006). [CrossRef]
  10. H. Y. Wang and C. Lu, "Electroporation of Mammalian Cells in a Microfluidic Channel with Geometric Variation," Anal. Chem. 78, 5158-5164 (2006). [CrossRef] [PubMed]
  11. D. C. Duffy, J. C. McDonald, O. J. A. Schueller, and G. M. Whitesides, "Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane)," Anal. Chem. 70, 4974-4984 (1998). [CrossRef] [PubMed]
  12. J. X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, "An epi-detected coherent anti-stokes Raman scattering microscope with high spectral resolution and high sensitivity," J. Phys. Chem. B 105, 1277-1280 (2001). [CrossRef]
  13. H. Wang, Y. Fu, P. Zickmund, R. Shi, and J. X. Cheng, "Coherent anti-stokes Raman scattering imaging of axonl myelin in live spinal tissues," Biophys. J. 89, 581-591 (2005). [CrossRef] [PubMed]
  14. A. Zumbusch, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering," Phys. Rev. Lett. 82, 4142-4145 (1999). [CrossRef]
  15. K. Bahlmann, P. T. So, M. Kirber, R. Reich, B. Kosicki, W. McGonagle, and K. Bellve, "Multifocal multiphoton microscopy (MMM) at a frame rate beyond 600 Hz," Opt. Express 15, 10991-10998 (2007). [CrossRef] [PubMed]
  16. K. H. Kim, C. Buehler, and P. T. C. So, "High-Speed, Two-Photon Scanning Microscope," Appl. Opt. 38, 6004-6009 (1999). [CrossRef]
  17. D. Huh, W. Gu, Y. Kamotani, J. B. Grotberg, and S. Takayama, "Microfluidics for flow cytometric analysis of cells and particles," Physiol. Meas. 26, R73-R98 (2005). [CrossRef] [PubMed]
  18. T. T. Le, I. M. Langohr, M. J. Locker, M. Sturek, and J. X. Cheng, "Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy," J. Biomed. Opt. 12, 054007 (2007). [CrossRef] [PubMed]
  19. Y. Fu, H. Wang, R. Shi, and J. X. Cheng, "Second harmonic and sum frequency generation imaging of fibrous astroglial filaments in ex vivo spinal tissues," Biophys. J. 92, 3251-3259 (2007). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited