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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 1 — Jan. 5, 2009
  • pp: 218–227

Nanodroplet real-time PCR system with laser assisted heating

Hanyoup Kim, Sanhita Dixit, Christopher J. Green, and Gregory W. Faris  »View Author Affiliations


Optics Express, Vol. 17, Issue 1, pp. 218-227 (2009)
http://dx.doi.org/10.1364/OE.17.000218


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Abstract

We report the successful application of low-power (~30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature measurement, and Taqman real-time readout in nanoliter droplets on a disposable plastic substrate. A selective heating scheme using an infrared laser appears ideal for driving PCR because it heats only the droplet, not the oil or plastic substrate, providing fast heating and completing the 40 cycles of PCR in 370 seconds. No microheaters or microfluidic circuitry were deposited on the substrate, and PCR was performed in one droplet without affecting neighboring droplets. The assay performance was quantitative and its amplification efficiency was comparable to that of a commercial instrument.

© 2009 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 11, 2008
Revised Manuscript: December 18, 2008
Manuscript Accepted: December 20, 2008
Published: December 24, 2008

Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Hanyoup Kim, Sanhita Dixit, Christopher J. Green, and Gregory W. Faris, "Nanodroplet real-time PCR system with laser assisted heating," Opt. Express 17, 218-227 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-1-218


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