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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 1 — Jan. 5, 2009
  • pp: 266–277

Optics InfoBase > Optics Express > Volume 17 > Issue 1 > Page 266

Optical diffraction tomography for high resolution live cell imaging

Yongjin Sung, Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations


Optics Express, Vol. 17, Issue 1, pp. 266-277 (2009)
http://dx.doi.org/10.1364/OE.17.000266


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Abstract

We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

© 2009 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 7, 2008
Revised Manuscript: November 20, 2008
Manuscript Accepted: December 11, 2008
Published: January 2, 2009

Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Yongjin Sung, Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Optical diffraction tomography for high resolution live cell imaging," Opt. Express 17, 266-277 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-1-266


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