OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 1 — Jan. 5, 2009
  • pp: 266–277

Optical diffraction tomography for high resolution live cell imaging

Yongjin Sung, Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations


Optics Express, Vol. 17, Issue 1, pp. 266-277 (2009)
http://dx.doi.org/10.1364/OE.17.000266


View Full Text Article

Enhanced HTML    Acrobat PDF (5036 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

© 2009 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 7, 2008
Revised Manuscript: November 20, 2008
Manuscript Accepted: December 11, 2008
Published: January 2, 2009

Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Yongjin Sung, Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Optical diffraction tomography for high resolution live cell imaging," Opt. Express 17, 266-277 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-1-266


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. F. Zernike, "Phase-contrast, a new method for microscopic observation of transparent objects. Part I.," Physica 9, 686-698 (1942). [CrossRef]
  2. Q1. G. Nomarski, "Microinterféromètre différentiel à ondes polarisées," J. Phys. Radium 16, 9S-11S (1955).
  3. Y. K. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, "Diffraction phase and fluorescence microscopy," Opt. Express 14, 8263-8268 (2006). [CrossRef] [PubMed]
  4. G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, "Diffraction phase microscopy for quantifying cell structure and dynamics," Opt. Lett. 31, 775-777 (2006). [CrossRef] [PubMed]
  5. C. Fang-Yen, S. Oh, Y. Park, W. Choi, S. Song, H. S. Seung, R. R. Dasari, and M. S. Feld, "Imaging voltage-dependent cell motions with heterodyne Mach-Zehnder phase microscopy," Opt. Lett. 32, 1572-1574 (2007). [CrossRef] [PubMed]
  6. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, "Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy," Opt. Lett. 30, 468-470 (2005). [CrossRef] [PubMed]
  7. A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, "Quantitative optical phase microscopy," Opt. Lett. 23, 817-819 (1998). [CrossRef]
  8. G. Popescu, T. Ikeda, K. Goda, C. A. Best-Popescu, M. Laposata, S. Manley, R. R. Dasari, K. Badizadegan, and M. S. Feld, "Optical measurement of cell membrane tension," Phys. Rev. Lett. 97, 218101 (2006). [CrossRef] [PubMed]
  9. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. Magistretti, "Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy," Opt. Express 13, 9361-9373 (2005). [CrossRef] [PubMed]
  10. N. Lue, G. Popescu, T. Ikeda, R. R. Dasari, K. Badizadegan, and M. S. Feld, "Live cell refractometry using microfluidic devices," Opt. Lett. 31, 2759-2761 (2006). [CrossRef] [PubMed]
  11. B. Rappaz, F. Charriere, C. Depeursinge, P. J. Magistretti, and P. Marquet, "Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium," Opt. Lett. 33, 744-746 (2008). [CrossRef] [PubMed]
  12. A. Barty, K. A. Nugent, A. Roberts, and D. Paganin, "Quantitative phase tomography," Opt. Commun. 175, 329-336 (2000). [CrossRef]
  13. W. Gorski and W. Osten, "Tomographic imaging of photonic crystal fibers," Opt. Lett. 32, 1977-1979 (2007). [CrossRef] [PubMed]
  14. F. Charriere, N. Pavillon, T. Colomb, C. Depeursinge, T. J. Heger, E. A. D. Mitchell, P. Marquet, and B. Rappaz, "Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba," Opt. Express 14, 7005-7013 (2006). [CrossRef] [PubMed]
  15. V. Lauer, "New approach to optical diffraction tomography yielding a vector equation of diffraction tomography and a novel tomographic microscope," J. Microsc. 205, 165-176 (2002). [CrossRef] [PubMed]
  16. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, "Tomographic phase microscopy," Nature Methods 4, 717-719 (2007). [CrossRef] [PubMed]
  17. K. C. Tam and V. Perezmendez, "Tomographical Imaging with Limited-Angle Input," J. Opt. Soc. Am. 71, 582-592 (1981). [CrossRef]
  18. B. P. Medoff, W. R. Brody, M. Nassi, and A. Macovski, "Iterative Convolution Backprojection Algorithms for Image-Reconstruction from Limited Data," J. Opt. Soc. Am. 73, 1493-1500 (1983). [CrossRef]
  19. A. C. Kak, and M. Slaney, Principles of Computerized Tomographic Imaging (Academic Press, New York, 1999).
  20. E. Wolf, "Three-dimensional structure determination of semi-transparent objects from holographic data," Opt. Commun. 1, 153-156 (1969). [CrossRef]
  21. Q2. M. Debailleul, B. Simon, V. Georges, O. Haeberle, and V. Lauer, "Holographic microscopy and diffractive microtomography of transparent samples," Meas. Sci. Technol. 19, (2008). [CrossRef]
  22. W. S. Choi, C. Fang-Yen, K. Badizadegan, R. R. Dasari, and M. S. Feld, "Extended depth of focus in tomographic phase microscopy using a propagation algorithm," Opt. Lett. 33, 171-173 (2008). [CrossRef] [PubMed]
  23. Y. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, "Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum," Proc. Natl. Acad. Sci. USA 105, 13730-13735 (2008). [CrossRef] [PubMed]
  24. W. Choi, C. C. Yu, C. Fang-Yen, K. Badizadegan, R. R. Dasari, and M. S. Feld, "Field-based angle-resolved light-scattering study of single live cells," Opt. Lett. 33, 1596-1598 (2008). [CrossRef] [PubMed]
  25. A. J. Devaney, "Inverse-Scattering Theory within the Rytov Approximation," Opt. Lett. 6, 374-376 (1981). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited