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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 10 — May. 11, 2009
  • pp: 7907–7915

Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues

Yicong Wu, Yuxin Leng, Jiefeng Xi, and Xingde Li  »View Author Affiliations


Optics Express, Vol. 17, Issue 10, pp. 7907-7915 (2009)
http://dx.doi.org/10.1364/OE.17.007907


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Abstract

An extremely compact all-fiber-optic scanning endomicroscopy system was developed for two-photon fluorescence (TPF) and second harmonic generation (SHG) imaging of biological samples. A conventional double-clad fiber (DCF) was employed in the endomicroscope for single-mode femtosecond pulse delivery, multimode nonlinear optical signals collection and fast two-dimensional scanning. A single photonic bandgap fiber (PBF) with negative group velocity dispersion at two-photon excitation wavelength (i.e. ~810 nm) was used for pulse prechirping in replacement of a bulky grating/lens -based pulse stretcher. The combined use of DCF and PBF in the endomicroscopy system made the endomicroscope basically a plug-and-play unit. The excellent imaging ability of the extremely compact all-fiber-optic nonlinear optical endomicroscopy system was demonstrated by SHG imaging of rat tail tendon and depth-resolved TPF imaging of epithelial tissues stained with acridine orange. The preliminary results suggested the promising potential of this extremely compact all-fiber-optic endomicroscopy system for realtime assessment of both epithelial and stromal structures in luminal organs.

© 2009 Optical Society of America

OCIS Codes
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(190.0190) Nonlinear optics : Nonlinear optics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 3, 2009
Revised Manuscript: April 21, 2009
Manuscript Accepted: April 25, 2009
Published: April 28, 2009

Virtual Issues
Vol. 4, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Yicong Wu, Yuxin Leng, Jiefeng Xi, and Xingde Li, "Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues," Opt. Express 17, 7907-7915 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-10-7907


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