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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 10 — May. 11, 2009
  • pp: 8264–8277

Experimental characterization of 3D localization techniques for particle-tracking and super-resolution microscopy

Michael J. Mlodzianoski, Manuel F. Juette, Glen L. Beane, and Joerg Bewersdorf  »View Author Affiliations


Optics Express, Vol. 17, Issue 10, pp. 8264-8277 (2009)
http://dx.doi.org/10.1364/OE.17.008264


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Abstract

Three-dimensional (3D) particle localization at the nanometer scale plays a central role in 3D particle tracking and 3D localization-based super-resolution microscopy. Here we introduce a localization algorithm that is independent of theoretical models and therefore generally applicable to a large number of experimental realizations. Applying this algorithm and a convertible experimental setup we compare the performance of the two major 3D techniques based on astigmatic distortions and on multiplane detection. In both methods we obtain experimental 3D localization accuracies in agreement with theoretical predictions and characterize the depth dependence of the localization accuracy in detail.

© 2009 Optical Society of America

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: March 6, 2009
Revised Manuscript: April 20, 2009
Manuscript Accepted: April 24, 2009
Published: April 30, 2009

Virtual Issues
Vol. 4, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Michael J. Mlodzianoski, Manuel F. Juette, Glen L. Beane, and Joerg Bewersdorf, "Experimental characterization of 3D localization techniques for particle-tracking and super-resolution microscopy," Opt. Express 17, 8264-8277 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-10-8264


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References

  1. J. Gelles, B. J. Schnapp, and M. P. Sheetz, "Tracking kinesin-driven movements with nanometre-scale precision," Nature 331, 450-453 (1988). [CrossRef] [PubMed]
  2. A. Yildiz, J. N. Forkey, S. A. McKinney, T. Ha, Y. E. Goldman, and P. R. Selvin, "Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization," Science 300, 2061-2065 (2003). [CrossRef] [PubMed]
  3. K. Murase, T. Fujiwara, Y. Umemura, K. Suzuki, R. Iino, H. Yamashita, M. Saito, H. Murakoshi, K. Ritchie, and A. Kusumi, "Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques," Biophys. J. 86, 4075-4093 (2004). [CrossRef] [PubMed]
  4. S. W. Hell, "Far-field optical nanoscopy," Science 316, 1153-1158 (2007). [CrossRef] [PubMed]
  5. S. W. Hell, "Microscopy and its focal switch," Nat. Methods 6, 24-32 (2009). [CrossRef] [PubMed]
  6. M. Speidel, A. Jonas, and E. L. Florin, "Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging," Opt. Lett. 28, 69-71 (2003). [CrossRef] [PubMed]
  7. M. Wu, J. W. Roberts, and M. Buckley, "Three-dimensional fluorescent particle tracking at micron-scale using a single camera," Exp. Fluids 38, 461-465 (2005). [CrossRef]
  8. H. P. Kao and A. S. Verkman, "Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position," Biophys. J. 67, 1291-1300 (1994). [CrossRef] [PubMed]
  9. L. Holtzer, T. Meckel, and T. Schmidt, "Nanometric three-dimensional tracking of individual quantum dots in cells," Appl. Phys. Lett. 90, 053902 (2007). [CrossRef]
  10. B. Huang, W. Wang, M. Bates, and X. Zhuang, "Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy," Science 319, 810-813 (2008). [CrossRef] [PubMed]
  11. P. Prabhat, S. Ram, E. S. Ward, and R. J. Ober, "Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions," IEEE Trans. Nanobiosci. 3, 237-242 (2004). [CrossRef]
  12. S. Ram, J. Chao, P. Prabhat, E. S. Ward, and R. J. Ober, "A novel approach to determining the three-dimensional location of microscopic objects with applications to 3D particle tracking," Proc. SPIE 6443, 1-7 (2007).
  13. P. Prabhat, Z. Gan, J. Chao, S. Ram, C. Vaccaro, S. Gibbons, R. J. Ober, and E. S. Ward, "Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy," Proc. Natl. Acad. Sci. USA 104, 5889-5894 (2007). [CrossRef] [PubMed]
  14. E. Toprak, H. Balci, B. H. Blehm, and P. R. Selvin, "Three-dimensional particle tracking via bifocal imaging," Nano Lett. 7, 2043-2045 (2007). [CrossRef] [PubMed]
  15. S. Ram, P. Prabhat, J. Chao, E. S. Ward, and R. J. Ober, "High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells," Biophys. J. 95, 6025-6043 (2008). [CrossRef] [PubMed]
  16. M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, "Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples," Nat. Methods 5, 527-529 (2008).
  17. http://www.nvidia.com/object/cuda_home.html.
  18. W. H. Press, S. A. Teukolsky, W. T. Vetterling, and B. P. Flannery, Numerical Recipes: The Art of Scientific Computing, 3rd ed., (Cambridge University Press, 2007).
  19. M. Frigo and S. G. Johnson, "FFTW," http://www.fftw.org/ (2008).
  20. S. Ram, E. S. Ward, and R. J. Ober, "How accurately can a single molecule be localized in three dimensions using a fluorescence microscope?," Proc. SPIE 5699, 426-435 (2005). [CrossRef] [PubMed]
  21. S. T. Hess, T. P. K. Girirajan, and M. D. Mason, "Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy," Biophys. J. 91, 4258-4272 (2006). [CrossRef] [PubMed]
  22. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, "Imaging intracellular fluorescent proteins at nanometer resolution," Science 313, 1642-1645 (2006). [CrossRef] [PubMed]
  23. C. von Middendorff, A. Egner, C. Geisler, S. W. Hell, and A. Schoenle, "Isotropic 3D Nanoscopy based on single emitter switching," Opt. Express 16, 20774-20788 (2008). [CrossRef] [PubMed]
  24. J. Enderlein, E. Toprak, and P. R. Selvin, "Polarization effect on position accuracy of fluorophore localization," Opt. Express 14, 8111-8120 (2006). [CrossRef] [PubMed]
  25. Y. Deng and J. W. Shaevitz, "Effect of aberration on height calibration in three-dimensional localization-based microscopy and particle tracking," Appl. Opt. 48, 1886-1890 (2009). [CrossRef] [PubMed]
  26. G. Shtengel, J. A. Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M. Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, "Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure," Proc. Natl. Acad. Sci. USA 106, 3125-3130 (2009). [CrossRef] [PubMed]
  27. S. R. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, "Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function," Proc. Natl. Acad. Sci. USA 106, 2995-2999 (2009). [CrossRef] [PubMed]

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