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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 11 — May. 25, 2009
  • pp: 8843–8855

Fluorescence lifetime imaging from time resolved measurements using a shape-based approach

Diego Álvarez, Paúl Medina, and Miguel Moscoso  »View Author Affiliations


Optics Express, Vol. 17, Issue 11, pp. 8843-8855 (2009)
http://dx.doi.org/10.1364/OE.17.008843


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Abstract

We present a novel fluorescent tomography algorithm to estimate the spatial distribution of fluorophores and the fluorescence lifetimes from surface time resolved measurements. The algorithm is a hybridization of the level set technique for recovering the distributions of distinct fluorescent markers with a gradient method for estimating their lifetimes. This imaging method offers several advantages compared to more traditional pixel-based techniques as, for example, well defined boundaries and a better resolution of the images. The numerical experiments show that our imaging method gives rise to accurate reconstructions in the presence of data noise and fluorescence background even for complicated fluorophore distributions in several-centimiter-thick biological tissue.

© 2009 Optical Society of America

OCIS Codes
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: April 6, 2009
Revised Manuscript: May 9, 2009
Manuscript Accepted: May 9, 2009
Published: May 12, 2009

Virtual Issues
Vol. 4, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Diego Álvarez, Paúl Medina, and Miguel Moscoso, "Fluorescence lifetime imaging from time resolved measurements using a shape-based approach," Opt. Express 17, 8843-8855 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-11-8843


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