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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 11 — May. 25, 2009
  • pp: 9235–9240

Second harmonic microscopy of axonemes

Christophe Odin, Claire Heichette, Denis Chretien, and Yann Le Grand  »View Author Affiliations


Optics Express, Vol. 17, Issue 11, pp. 9235-9240 (2009)
http://dx.doi.org/10.1364/OE.17.009235


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Abstract

We performed Second Harmonic Microscopy of axonemes obtained from sea urchin sperm. Using polarization analysis and a trade-off between signal and photodamage, we were able to determine, for the first time to our knowledge, the nonlinear susceptibility χzxx/χxzx = 1.1±0.2 and χzzz/χxzx = 4±0.5 of axonemes.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(160.1435) Materials : Biomaterials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 18, 2009
Revised Manuscript: May 13, 2009
Manuscript Accepted: May 13, 2009
Published: May 18, 2009

Virtual Issues
Vol. 4, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Christophe Odin, Claire Heichette, Denis Chretien, and Yann Le Grand, "Second harmonic microscopy of axonemes," Opt. Express 17, 9235-9240 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-11-9235


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