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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 11 — May. 25, 2009
  • pp: 9235–9240

Second harmonic microscopy of axonemes

Christophe Odin, Claire Heichette, Denis Chretien, and Yann Le Grand  »View Author Affiliations

Optics Express, Vol. 17, Issue 11, pp. 9235-9240 (2009)

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We performed Second Harmonic Microscopy of axonemes obtained from sea urchin sperm. Using polarization analysis and a trade-off between signal and photodamage, we were able to determine, for the first time to our knowledge, the nonlinear susceptibility χzxx /χxzx = 1.1 ± 0.2 and χzzz /χxzx = 4± 0.5 of axonemes.

© 2009 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(160.1435) Materials : Biomaterials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: March 18, 2009
Revised Manuscript: May 13, 2009
Manuscript Accepted: May 13, 2009
Published: May 18, 2009

Virtual Issues
Vol. 4, Iss. 7 Virtual Journal for Biomedical Optics

Christophe Odin, Claire Heichette, Denis Chretien, and Yann Le Grand, "Second harmonic microscopy of axonemes," Opt. Express 17, 9235-9240 (2009)

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