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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 12 — Jun. 8, 2009
  • pp: 10098–10104

A 0.4-mm-diameter probe for nonlinear optical imaging

Hongchun Bao and Min Gu  »View Author Affiliations


Optics Express, Vol. 17, Issue 12, pp. 10098-10104 (2009)
http://dx.doi.org/10.1364/OE.17.010098


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Abstract

A miniaturized probe that possesses a diameter of 0.4 mm is developed for two-photon-excited fluorescence imaging. The miniaturized probe was manufactured by the collapse of air holes and the formation of a lens on the tip of a double-clad photonic crystal fiber (DCPCF) using electric arc discharging from a conventional fusion splicer. As a result, a femtosecond pulsed laser beam delivered by the DCPCF can be directly focused on a sample for two-photon fluorescence imaging. The numerical aperture of the lensed DCPCF is 0.12. The corresponding focal spot size is 6 µm, which is close to the diffraction limit. This 0.4-mm-diamter probe can provide clear two-photon-excited fluorescence images of 10-µm-diameter fluorescent microspheres.

© 2009 OSA

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(110.3080) Imaging systems : Infrared imaging
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: March 20, 2009
Revised Manuscript: May 27, 2009
Manuscript Accepted: May 27, 2009
Published: June 1, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Hongchun Bao and Min Gu, "A 0.4-mm-diameter probe for nonlinear optical imaging," Opt. Express 17, 10098-10104 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-12-10098


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