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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 14 — Jul. 6, 2009
  • pp: 11294–11308

A field theoretical restoration method for images degraded by non-uniform light attenuation : an application for light microscopy

Hwee Kuan Lee, Mohammad Shorif Uddin, Shvetha Sankaran, Srivats Hariharan, and Sohail Ahmed  »View Author Affiliations

Optics Express, Vol. 17, Issue 14, pp. 11294-11308 (2009)

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Microscopy has become a de facto tool for biology. However, it suffers from a fundamental problem of poor contrast with increasing depth, as the illuminating light gets attenuated and scattered and hence can not penetrate through thick samples. The resulting decay of light intensity due to attenuation and scattering varies exponentially across the image. The classical space invariant deconvolution approaches alone are not suitable for the restoration of uneven illumination in microscopy images. In this paper, we present a novel physics-based field theoretical approach to solve the contrast degradation problem of light microscopy images. We have confirmed the effectiveness of our technique through simulations as well as through real field experimentations.

© 2009 Optical Society of America

OCIS Codes
(000.3860) General : Mathematical methods in physics
(100.2000) Image processing : Digital image processing
(100.3020) Image processing : Image reconstruction-restoration
(110.0180) Imaging systems : Microscopy

ToC Category:
Imaging Systems

Original Manuscript: April 29, 2009
Revised Manuscript: June 16, 2009
Manuscript Accepted: June 18, 2009
Published: June 22, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Hwee K. Lee, Mohammad S. Uddin, Shvetha Sankaran, Srivats Hariharan, and Sohail Ahmed, "A field theoretical restoration method for images degraded by non-uniform light attenuation : an application for light microscopy," Opt. Express 17, 11294-11308 (2009)

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