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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 14 — Jul. 6, 2009
  • pp: 12013–12018

Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence detected IR spectroscopy

Keiichi Inoue, Nándor Bokor, Satoshi Kogure, Masaaki Fujii, and Makoto Sakai  »View Author Affiliations


Optics Express, Vol. 17, Issue 14, pp. 12013-12018 (2009)
http://dx.doi.org/10.1364/OE.17.012013


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Abstract

IR absorption of chemical species in microscopic objects such as biological cells cannot be measured by conventional IR microscopes, because of their low resolution. To overcome this problem, we developed a novel far-field IR super-resolution microscope employing transient fluorescence detected IR spectroscopy. The resolution of this microscope was shown to be 880 nm by measuring the image of 1 µm fluorescent beads. Furthermore, it succeeded in resolving beads located 1.4 µm apart from each other. This is considerably smaller than the diffraction limit of the applied IR light (3.4 µm). These results suggest the capability of our microscope to study sub-micron targets such as sub-cellular structures of biological cells.

© 2009 OSA

OCIS Codes
(110.3080) Imaging systems : Infrared imaging
(130.3060) Integrated optics : Infrared
(170.0110) Medical optics and biotechnology : Imaging systems
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(320.5390) Ultrafast optics : Picosecond phenomena

ToC Category:
Microscopy

History
Original Manuscript: April 23, 2009
Revised Manuscript: June 15, 2009
Manuscript Accepted: June 24, 2009
Published: July 1, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Keiichi Inoue, Nándor Bokor, Satoshi Kogure, Masaaki Fujii, and Makoto Sakai, "Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence detected IR spectroscopy," Opt. Express 17, 12013-12018 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-14-12013


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References

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