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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 15 — Jul. 20, 2009
  • pp: 12502–12509

Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation

Bevin Lin, Shiro Urayama, Ramez M. G. Saroufeem, Dennis L. Matthews, and Stavros G. Demos  »View Author Affiliations

Optics Express, Vol. 17, Issue 15, pp. 12502-12509 (2009)

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Detection of esophageal disease in current clinical practice is limited to visualization of macroscopic epithelial morphology. In this work, we investigate high resolution autofluorescence imaging under ultra violet excitation to visualize microscopic epithelial changes related to disease progression using a bench top prototype microscope. The approach is based on the hypothesis that UV excitation light can only penetrate the superficial layer of cells resulting in autofluorescence images of the epithelial layer without using an additional image sectioning approach. The experiments were performed using ex vivo human esophagus biopsy specimens. The results indicate that cellular morphology information related to disease progression is attainable without tissue preparation.

© 2009 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.2680) Medical optics and biotechnology : Gastrointestinal
(170.4730) Medical optics and biotechnology : Optical pathology
(260.7190) Physical optics : Ultraviolet
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 29, 2009
Revised Manuscript: July 2, 2009
Manuscript Accepted: July 5, 2009
Published: July 8, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Bevin Lin, Shiro Urayama, Ramez M. G. Saroufeem, Dennis L. Matthews, and Stavros G. Demos, "Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation," Opt. Express 17, 12502-12509 (2009)

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