Abstract

Phase imaging with a high-resolution wavefront sensor is considered. This is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. The measurement technology is explained both in the scope of wave optics and geometrical optics in order to discuss its implementation on a conventional microscope. In particular we consider the effect of a non spatially coherent source on the phase-image signal-to-noise ratio. Precise measurements of the phase-shift introduced by microscopic beads or giant unilamellar vesicles validate the principle and show the accuracy of the methods. Diffraction limited images of living COS-7 cells are then presented, with a particular focus on the membrane and organelle dynamics.

© 2009 Optical Society of America

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History
Original Manuscript: April 24, 2009
Manuscript Accepted: July 6, 2009
Revised Manuscript: July 3, 2009
Published: July 16, 2009

References

1. F. Zernike, "Das Phasenkontrastverfahren bei der mikroskopischen Beobachtung," Z. Tech. Phys. 16, 454-457 (1935).
2. G. Nomarski, "Nouveau Dispositif Pour l’Observation En Contraste De Phase Differentiel," J. Phys. Radium 16, S88-S88 (1955).
3. P. Hariharan, "Achromatic Phase-Shifting for White-Light Interferometry," Appl. Opt. 35(34), 6823-6824 (1996). [CrossRef]
4. C. J. Cogswell, N. I. Smith, K. G. Larkin and P. Hariharan, "Quantitative DIC microscopy using a geometric phase shifter," Society of Photo-Optical Instrumentation Engineers (SPIE) Conference Series, C. J. Cogswell, J. A. Conchello and T. Wilson, eds. (1997), pp. 72-81.
5. Y. Xu, Y. x. Xu, M. Hui and X. Cai, "Quantitative surface topography determination by differential interference contrast microscopy," Opt. Precision Eng. 9, 226-229 (2001).
6. M. R. Arnison, K. G. Larkin, C. J. R. Sheppard, N. I. Smith, and C. J. Cogswell, "Linear phase imaging using differential interference contrast microscopy," J. Microscopy 214(1), 7-12 (2004). [CrossRef]
7. S. Bernet, A. Jesacher, S. Fürhapter, C. Maurer and M. Ritsch-Marte, "Quantitative imaging of complex samples by spiral phase contrast microscopy," Opt. Express 14(9), 3792-3805 (2006). [CrossRef]
8. E. Cuche, F. Bevilacqua and C. Depeursinge, "Digital holography for quantitative phase-contrast imaging," Opt. Lett. 24(5), 291-293 (1999). [CrossRef]
9. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb and C. Depeursinge, "Digital holographic microscopy: a noninvasive contrastimaging technique allowing quantitative visualization ofliving cells with subwavelength axial accuracy," Opt. Lett. 30(5), 468-470 (2005). [CrossRef]
10. F. Charrière, A. Marian, F. Montfort, J. Kuehn, T. Colomb, E. Cuche, P. Marquet and C. Depeursinge, "Cell refractive index tomography by digital holographic microscopy," Opt. Lett. 31(2), 178-180 (2006). [CrossRef]
11. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari and M. S. Feld, "Tomographic phase microscopy," Nature Methods 4, 717-719 (2007). [CrossRef]
12. M. Born and E. Wolf, Principles of Optics, (Cambridge University Press, 1999).
13. J. Zou, A. Sautivet, J. Fils, L. Martin, K. Abdeli, C. Sauteret and B. Wattellier, "Optimization of the dynamic wavefront control of a pulsed kilojoule/nanosecond-petawatt laser facility," Appl. Opt. 47(5), 704-710 (2008). [CrossRef]
14. R. V. Shack and B. C. Platt, "Production and use of a lenticular Hartmann screen," J. Opt. Soc. Am. 61, 656 (1971).
15. A. Barty, K. A. Nugent, D. Paganin and A. Roberts, "Quantitative optical phase microscopy," Opt. Lett. 23(11), 817-819 (1998). [CrossRef]
16. C. J. Bellair, C. L. Curl, B. E. Allman, P. J. Harris, A. Roberts and L. M. D. Delbridge, "Quantitative phase amplitude microscopy IV: imaging thick specimens," J. Microscopy 214(1), 62-68 (2004). [CrossRef]
17. C. Curl, C. J. Bellair, P. J. Harris, B. E. Allman, A. Roberts, K. A. Nugent and L. M. D. Delbridge, "Single cell volume measurement by Quantitative Phase Microscopy (QPM): A case study of Erythrocyte Morphology," Cellular Physiology Biochem. 17, 193-200 (2006). [CrossRef]
18. J. Primot and L. Sogno, "Achromatic three-wave (or more) lateral shearing interferometer," J. Opt. Soc. Am. A 12(12), 2679 (1995). [CrossRef]
19. J. Primot, "Theoretical description of Shack-Hartmann wave-front sensor," Opt. Commun. 222, 81-92 (2003). [CrossRef]
20. J. Primot and N. Guérineau, "Extended Hartmann Test Based on the Pseudoguiding Property of a Hartmann Mask Completed by a Phase Chessboard," Appl. Opt. 39(31), 5715-5720 (2000). [CrossRef]
21. S. Velghe, J. Primot, N. Guérineau, R. Haidar, S. Demoustier, M. Cohen and B. Wattellier, "Advanced wave-front sensing by quadri-wave lateral shearing interferometry," Proc. SPIE 6292, 62920E (2006).
22. M. J. Weber, Handbook of Optical materials, (CRC Press, 2003).
23. M. Angelova, S. Soléau, P. Méléard and P. Bothorel, "Preparation of giant vesicles by external AC electric fields. Kinetics and applications," in Trends in Colloid and Interface Science V, Steinkopff Darmstadt, eds., (1992), pp. 127-131.
24. J. F. Nagle and S. Tristram-Nagle, "Structure of lipid bilayers," Biochim. Biophys. Acta 1469(3), 159-195 (2000).
25. C. S. Chong and C. Colbow, "Light scattering and turbidity measurements on lipid vesicles," Biochim. Biophys. Acta 436,260-282 (1976). [CrossRef]
26. F. C. Jensen, J. Anthony, R. V. G. Girardi and H. Koprowski (1964), "Infection of Human and Simian Tissue Cultures with Rous Sarcoma Virus," PNAS 52(1), 53-59 (1964). [CrossRef]
27. A. Sergé, N. Bertaux, H. Rigneault and D. Marguet, "Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes," Nat. Methods 5, 687-695 (2008). [CrossRef]

Author Affiliations

Serge Monneret

Institut fresnel

Benoit Wattellier

Phasics

Guillaume Maucort

Phasics / Institut Fresnel

Pierre Bon

Phasics / Institut Fresnel UMR6133

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