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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 15 — Jul. 20, 2009
  • pp: 13255–13269

Quantifying Noise in Optical Tweezers by Allan Variance

Fabian Czerwinski, Andrew C. Richardson, and Lene B. Oddershede  »View Author Affiliations

Optics Express, Vol. 17, Issue 15, pp. 13255-13269 (2009)

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Much effort is put into minimizing noise in optical tweezers experiments because noise and drift can mask fundamental behaviours of, e.g., single molecule assays. Various initiatives have been taken to reduce or eliminate noise but it has been difficult to quantify their effect. We propose to use Allan variance as a simple and efficient method to quantify noise in optical tweezers setups. We apply the method to determine the optimal measurement time, frequency, and detection scheme, and quantify the effect of acoustic noise in the lab. The method can also be used on-the-fly for determining optimal parameters of running experiments.

© 2009 Optical Society of America

OCIS Codes
(000.3110) General : Instruments, apparatus, and components common to the sciences
(000.5490) General : Probability theory, stochastic processes, and statistics
(030.4280) Coherence and statistical optics : Noise in imaging systems
(110.4280) Imaging systems : Noise in imaging systems
(140.7010) Lasers and laser optics : Laser trapping
(350.4800) Other areas of optics : Optical standards and testing

ToC Category:
Optical Tweezers or Optical Manipulation

Original Manuscript: July 1, 2009
Revised Manuscript: July 15, 2009
Manuscript Accepted: July 15, 2009
Published: July 17, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Fabian Czerwinski, Andrew C. Richardson, and Lene B. Oddershede, "Quantifying Noise in Optical Tweezers by Allan Variance," Opt. Express 17, 13255-13269 (2009)

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