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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 16 — Aug. 3, 2009
  • pp: 13381–13388

Light scattering characterization of mitochondrial aggregation in single cells

Xuan-Tao Su, Kirat Singh, Wojciech Rozmus, Christopher Backhouse, and Clarence Capjack  »View Author Affiliations


Optics Express, Vol. 17, Issue 16, pp. 13381-13388 (2009)
http://dx.doi.org/10.1364/OE.17.013381


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Abstract

Three dimensional finite-difference time-domain (FDTD) simulations are employed to show that light scattering techniques may be used to infer the mitochondrial distributions that exist within single biological cells. Two-parameter light scattering plots of the FDTD light scattering spectra show that the small angle forward scatter can be used to differentiate the case of a random distribution of mitochondria within a cell model from that in which the mitochondria are aggregated to the nuclear periphery. Fourier transforms of the wide angle side scatter spectra show a consistent highest dominant frequency, which may be used for size differentiation of biological cells with distributed mitochondria.

© 2009 OSA

OCIS Codes
(000.1430) General : Biology and medicine
(000.4430) General : Numerical approximation and analysis
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1610) Medical optics and biotechnology : Clinical applications
(290.0290) Scattering : Scattering

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 21, 2008
Revised Manuscript: June 1, 2009
Manuscript Accepted: July 15, 2009
Published: July 20, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Xuan-Tao Su, Kirat Singh, Wojciech Rozmus, Christopher Backhouse, and Clarence Capjack, "Light scattering characterization of mitochondrial aggregation in single cells," Opt. Express 17, 13381-13388 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-16-13381


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