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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 16 — Aug. 3, 2009
  • pp: 13447–13457

Detecting intrinsic scattering changes correlated to neuron action potentials using optical coherence imaging

Benedikt W. Graf, Tyler S. Ralston, Han-Jo Ko, and Stephen A. Boppart  »View Author Affiliations


Optics Express, Vol. 17, Issue 16, pp. 13447-13457 (2009)
http://dx.doi.org/10.1364/OE.17.013447


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Abstract

We demonstrate how optical coherence imaging techniques can detect intrinsic scattering changes that occur during action potentials in single neurons. Using optical coherence tomography (OCT), an increase in scattering intensity from neurons in the abdominal ganglion of Aplysia californica is observed following electrical stimulation of the connective nerve. In addition, optical coherence microscopy (OCM), with its superior transverse spatial resolution, is used to demonstrate a direct correlation between scattering intensity changes and membrane voltage in single cultured Aplysia bag cell neurons during evoked action potentials. While intrinsic scattering changes are small, OCT and OCM have potential use as tools in neuroscience research for non-invasive and non-contact measurement of neural activity without electrodes or fluorescent dyes. These techniques have many attractive features such as high sensitivity and deep imaging penetration depth, as well as high temporal and spatial resolution. This study demonstrates the first use of OCT and OCM to detect functionally-correlated optical scattering changes in single neurons.

© 2009 OSA

OCIS Codes
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 1, 2009
Revised Manuscript: July 13, 2009
Manuscript Accepted: July 15, 2009
Published: August 3, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Benedikt W. Graf, Tyler S. Ralston, Han-Jo Ko, and Stephen A. Boppart, "Detecting intrinsic scattering changes 
correlated to neuron action potentials 
using optical coherence imaging," Opt. Express 17, 13447-13457 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-16-13447


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