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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 17 — Aug. 17, 2009
  • pp: 14534–14542

Fourier transform-second-harmonic generation imaging of biological tissues

Raghu Ambekar Ramachandra Rao, Monal R. Mehta, and Kimani C. Toussaint, Jr.  »View Author Affiliations


Optics Express, Vol. 17, Issue 17, pp. 14534-14542 (2009)
http://dx.doi.org/10.1364/OE.17.014534


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Abstract

Fourier transform-second-harmonic generation imaging is employed to obtain quantitative metrics of collagen fibers in biological tissues. In particular, the preferred orientation and maximum spatial frequency of collagen fibers for selected regions of interest in porcine trachea, ear, and cornea are determined. These metrics remain consistent when applied to collagen fibers in the ear, which can be expected from observation. Collagen fibers in the trachea are more random with large standard deviations in orientation, and large variations in maximum spatial frequency. In addition, these metrics are used to investigate structural changes through a 3D stack of the cornea. This technique can be used as a quantitative marker to assess the structure of collagen fibers that may change due to damage from disease or physical injury.

© 2009 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: June 16, 2009
Revised Manuscript: July 30, 2009
Manuscript Accepted: July 31, 2009
Published: August 3, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Raghu A. Rao, Monal R. Mehta, and Kimani C. Toussaint, "Fourier transform-second-harmonic generation imaging of biological tissues," Opt. Express 17, 14534-14542 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-17-14534


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