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Ultra high-speed swept source OCT imaging of the anterior segment of human eye at 200 kHz with adjustable imaging range
Michalina Gora, Karol Karnowski, Maciej Szkulmowski, Bartlomiej J. Kaluzny, Robert Huber, Andrzej Kowalczyk, and Maciej Wojtkowski »View Author Affiliations
1Institute of Physics, Nicolaus Copernicus University, ul. Grudziądzka 5/7, PL-87-100 Toruń, Poland
2Collegium Medicum, Nicolaus Copernicus University, ul. Curie-Skłodowskiej 9, PL-85-094 Bydgoszcz, Poland
3Institute of Physics, Ludwig Maximilians University, Oettingenstr. 67, 80538 Munich, Germany
*Corresponding author: Maciej.Wojtkowski@fizyka.umk.pl
Optics Express, Vol. 17, Issue 17, pp. 14880-14894 (2009)
http://dx.doi.org/10.1364/OE.17.014880
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Abstract
We present an application of in vivo anterior segment imaging of the human eye with an ultrahigh speed swept source OCT instrument. For this purpose, a dedicated OCT system was designed and constructed. This instrument enables axial zooming by automatic reconfiguration of spectral sweep range; an enhanced imaging range mode enables imaging of the entire anterior segment while a high axial resolution mode provides detailed morphological information of the chamber angle and the cornea. The speed of 200,000 lines/s enables high sampling density in three-dimensional imaging of the entire cornea in 250 ms promising future applications of OCT for optical corneal topography, pachymetry and elevation maps. The results of a preliminary quantitative corneal analysis based on OCT data free form motion artifacts are presented. Additionally, a volumetric and real time reconstruction of dynamic processes, like pupillary reaction to light stimulus or blink-induced contact lens movements are demonstrated.
© 2009 OSA
OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(140.3600) Lasers and laser optics : Lasers, tunable
(170.4470) Medical optics and biotechnology : Ophthalmology
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: June 15, 2009
Revised Manuscript: July 31, 2009
Manuscript Accepted: August 4, 2009
Published: August 6, 2009
Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics
Citation
Michalina Gora, Karol Karnowski, Maciej Szkulmowski, Bartlomiej J. Kaluzny, Robert Huber, Andrzej Kowalczyk, and Maciej Wojtkowski, "Ultra high-speed swept source OCT imaging of the anterior segment of human eye at 200 kHz with adjustable imaging range," Opt. Express 17, 14880-14894 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-17-14880
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- S. Radhakrishnan, J. Goldsmith, D. Huang, V. Westphal, D. K. Dueker, A. M. Rollins, J. A. Izatt, and S. D. Smith, “Comparison of optical coherence tomography and ultrasound biomicroscopy for detection of narrow anterior chamber angles,” Arch. Ophthalmol. 123(8), 1053–1059 (2005). [PubMed]
- V. Christopoulos, L. Kagemann, G. Wollstein, H. Ishikawa, M. L. Gabriele, M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, J. S. Duker, D. K. Dhaliwal, and J. S. Schuman, “In vivo corneal high-speed, ultra high-resolution optical coherence tomography,” Arch. Ophthalmol. 125(8), 1027–1035 (2007). [PubMed]
- M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, T. Ko, J. S. Schuman, A. Kowalczyk, and J. S. Duker, “Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography,” Ophthalmology 112(10), 1734–1746 (2005). [PubMed]
- B. R. Biedermann, W. Wieser, C. M. Eigenwillig, T. Klein, and R. Huber, “Dispersion, coherence and noise of Fourier domain mode locked lasers,” Opt. Express . 17, 9947-9961 (2009). [PubMed]
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- E. Götzinger, M. Pircher, M. Sticker, A. F. Fercher, and C. K. Hitzenberger, “Measurement and imaging of birefringent properties of the human cornea with phase-resolved, polarization-sensitive optical coherence tomography,” J. Biomed. Opt. 9(1), 94–102 (2004). [PubMed]
- A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography-principles and applications,” Rep. Prog. Phys. 66, 239–303 (2003).
- R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of fourier domain vs. time domain optical coherence tomography,” Opt. Express 11(8), 889–894 (2003). [PubMed]
- M. Wojtkowski, R. Leitgeb, A. Kowalczyk, T. Bajraszewski, and A. F. Fercher, “In vivo human retinal imaging by Fourier domain optical coherence tomography,” J. Biomed. Opt. 7(3), 457–463 (2002). [PubMed]
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- B. J. Kaluzny, W. Fojt, A. Szkulmowska, T. Bajraszewski, M. Wojtkowski, and A. Kowalczyk, “Spectral optical coherence tomography in video-rate and 3D imaging of contact lens wear,” Optom. Vis. Sci. 84(12), 1104–1109 (2007). [PubMed]
- S. Radhakrishnan, J. See, S. D. Smith, W. P. Nolan, Z. Ce, D. S. Friedman, D. Huang, Y. Li, T. Aung, and P. T. K. Chew, “Reproducibility of anterior chamber angle measurements obtained with anterior segment optical coherence tomography,” Invest. Ophthalmol. Vis. Sci. 48(8), 3683–3688 (2007). [PubMed]
- V. Christopoulos, L. Kagemann, G. Wollstein, H. Ishikawa, M. L. Gabriele, M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, J. S. Duker, D. K. Dhaliwal, and J. S. Schuman, “In vivo corneal high-speed, ultra high-resolution optical coherence tomography,” Arch. Ophthalmol. 125(8), 1027–1035 (2007). [PubMed]
- R. Huber, D. C. Adler, and J. G. Fujimoto, “Buffered Fourier domain mode locking: Unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s,” Opt. Lett. 31(20), 2975–2977 (2006). [PubMed]
- R. Huber, M. Wojtkowski, and J. G. Fujimoto, “Fourier Domain Mode Locking (FDML): A new laser operating regime and applications for optical coherence tomography,” Opt. Express 14(8), 3225–3237 (2006). [PubMed]
- R. Huber, M. Wojtkowski, J. G. Fujimoto, J. Y. Jiang, and A. E. Cable, “Three-dimensional and C-mode OCT imaging with a compact, frequency swept laser source at 1300 nm,” Opt. Express 13(26), 10523–10538 (2005). [PubMed]
- M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, T. Ko, J. S. Schuman, A. Kowalczyk, and J. S. Duker, “Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography,” Ophthalmology 112(10), 1734–1746 (2005). [PubMed]
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Gora, M.
- I. Grulkowski, M. Gora, M. Szkulmowski, I. Gorczynska, D. Szlag, S. Marcos, A. Kowalczyk, and M. Wojtkowski, “Anterior segment imaging with Spectral OCT system using a high-speed CMOS camera,” Opt. Express 17(6), 4842–4858 (2009). [PubMed]
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- E. Götzinger, M. Pircher, M. Sticker, A. F. Fercher, and C. K. Hitzenberger, “Measurement and imaging of birefringent properties of the human cornea with phase-resolved, polarization-sensitive optical coherence tomography,” J. Biomed. Opt. 9(1), 94–102 (2004). [PubMed]
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Hitzenberger, C. K.
- E. Götzinger, M. Pircher, I. Dejaco-Ruhswurm, S. Kaminski, C. Skorpik, and C. K. Hitzenberger, “Imaging of birefringent properties of keratoconus corneas by polarization-sensitive optical coherence tomography,” Invest. Ophthalmol. Vis. Sci. 48(8), 3551–3558 (2007). [PubMed]
- E. Götzinger, M. Pircher, M. Sticker, A. F. Fercher, and C. K. Hitzenberger, “Measurement and imaging of birefringent properties of the human cornea with phase-resolved, polarization-sensitive optical coherence tomography,” J. Biomed. Opt. 9(1), 94–102 (2004). [PubMed]
- A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography-principles and applications,” Rep. Prog. Phys. 66, 239–303 (2003).
- R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of fourier domain vs. time domain optical coherence tomography,” Opt. Express 11(8), 889–894 (2003). [PubMed]
- A. Konstantopoulos, P. Hossain, and D. F. Anderson, “Recent advances in ophthalmic anterior segment imaging: a new era for ophthalmic diagnosis?” Br. J. Ophthalmol. 91(4), 551–557 (2007). [PubMed]
- M. A. Choma, K. Hsu, and J. A. Izatt, “Swept source optical coherence tomography using an all-fiber 1300-nm ring laser source,” J. Biomed. Opt. 10, 044009 (2005).
- R. Huber, M. Wojtkowski, K. Taira, J. Fujimoto, and K. Hsu, “Amplified, frequency swept lasers for frequency domain reflectometry and OCT imaging: design and scaling principles,” Opt. Express 13(9), 3513–3528 (2005). [PubMed]
- S. Radhakrishnan, J. See, S. D. Smith, W. P. Nolan, Z. Ce, D. S. Friedman, D. Huang, Y. Li, T. Aung, and P. T. K. Chew, “Reproducibility of anterior chamber angle measurements obtained with anterior segment optical coherence tomography,” Invest. Ophthalmol. Vis. Sci. 48(8), 3683–3688 (2007). [PubMed]
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- J. A. Izatt, M. R. Hee, E. A. Swanson, C. P. Lin, D. Huang, J. S. Schuman, C. A. Puliafito, and J. G. Fujimoto, “Micrometer-scale resolution imaging of the anterior eye in vivo with optical coherence tomography,” Arch. Ophthalmol. 112(12), 1584–1589 (1994). [PubMed]
- D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991). [PubMed]
- B. R. Biedermann, W. Wieser, C. M. Eigenwillig, T. Klein, and R. Huber, “Dispersion, coherence and noise of Fourier domain mode locked lasers,” Opt. Express . 17, 9947-9961 (2009). [PubMed]
- T. Bajraszewski, M. Wojtkowski, M. Szkulmowski, A. Szkulmowska, R. Huber, and A. Kowalczyk, “Improved spectral optical coherence tomography using optical frequency comb,” Opt. Express 16(6), 4163–4176 (2008). [PubMed]
- C. M. Eigenwillig, B. R. Biedermann, G. Palte, and R. Huber, “K-space linear Fourier domain mode locked laser and applications for optical coherence tomography,” Opt. Express 16(12), 8916–8937 (2008). [PubMed]
- R. Huber, D. C. Adler, and J. G. Fujimoto, “Buffered Fourier domain mode locking: Unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s,” Opt. Lett. 31(20), 2975–2977 (2006). [PubMed]
- R. Huber, M. Wojtkowski, and J. G. Fujimoto, “Fourier Domain Mode Locking (FDML): A new laser operating regime and applications for optical coherence tomography,” Opt. Express 14(8), 3225–3237 (2006). [PubMed]
- R. Huber, M. Wojtkowski, K. Taira, J. Fujimoto, and K. Hsu, “Amplified, frequency swept lasers for frequency domain reflectometry and OCT imaging: design and scaling principles,” Opt. Express 13(9), 3513–3528 (2005). [PubMed]
- R. Huber, M. Wojtkowski, J. G. Fujimoto, J. Y. Jiang, and A. E. Cable, “Three-dimensional and C-mode OCT imaging with a compact, frequency swept laser source at 1300 nm,” Opt. Express 13(26), 10523–10538 (2005). [PubMed]
- V. Christopoulos, L. Kagemann, G. Wollstein, H. Ishikawa, M. L. Gabriele, M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, J. S. Duker, D. K. Dhaliwal, and J. S. Schuman, “In vivo corneal high-speed, ultra high-resolution optical coherence tomography,” Arch. Ophthalmol. 125(8), 1027–1035 (2007). [PubMed]
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- M. A. Choma, K. Hsu, and J. A. Izatt, “Swept source optical coherence tomography using an all-fiber 1300-nm ring laser source,” J. Biomed. Opt. 10, 044009 (2005).
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- V. Christopoulos, L. Kagemann, G. Wollstein, H. Ishikawa, M. L. Gabriele, M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, J. S. Duker, D. K. Dhaliwal, and J. S. Schuman, “In vivo corneal high-speed, ultra high-resolution optical coherence tomography,” Arch. Ophthalmol. 125(8), 1027–1035 (2007). [PubMed]
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- B. J. Kaluzny, B. J. Kaluzy, J. J. Kałuzny, A. Szkulmowska, I. Gorczyńska, M. Szkulmowski, T. Bajraszewski, M. Wojtkowski, and P. Targowski, “Spectral optical coherence tomography: a novel technique for cornea imaging,” Cornea 25(8), 960–965 (2006). [PubMed]
- E. Götzinger, M. Pircher, I. Dejaco-Ruhswurm, S. Kaminski, C. Skorpik, and C. K. Hitzenberger, “Imaging of birefringent properties of keratoconus corneas by polarization-sensitive optical coherence tomography,” Invest. Ophthalmol. Vis. Sci. 48(8), 3551–3558 (2007). [PubMed]
- S. Fukuda, K. Kawana, Y. Yasuno, and T. Oshika, “Anterior ocular biometry using 3-dimensional optical coherence tomography,” Ophthalmology 116(5), 882–889 (2009). [PubMed]
- M. Miura, K. Kawana, T. Iwasaki, T. Kiuchi, T. Oshika, H. Mori, M. Yamanari, S. Makita, T. Yatagai, and Y. Yasuno, “Three-dimensional anterior segment optical coherence tomography of filtering blebs after trabeculectomy,” J. Glaucoma 17(3), 193–196 (2008). [PubMed]
- M. Miura, K. Kawana, T. Iwasaki, T. Kiuchi, T. Oshika, H. Mori, M. Yamanari, S. Makita, T. Yatagai, and Y. Yasuno, “Three-dimensional anterior segment optical coherence tomography of filtering blebs after trabeculectomy,” J. Glaucoma 17(3), 193–196 (2008). [PubMed]
- C. K. Leung, W. M. Chan, C. Y. Ko, S. I. Chui, J. Woo, M. K. Tsang, and R. K. Tse, “Visualization of anterior chamber angle dynamics using optical coherence tomography,” Ophthalmology 112(6), 980–984 (2005). [PubMed]
- M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, T. Ko, J. S. Schuman, A. Kowalczyk, and J. S. Duker, “Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography,” Ophthalmology 112(10), 1734–1746 (2005). [PubMed]
- A. Konstantopoulos, P. Hossain, and D. F. Anderson, “Recent advances in ophthalmic anterior segment imaging: a new era for ophthalmic diagnosis?” Br. J. Ophthalmol. 91(4), 551–557 (2007). [PubMed]
- I. Grulkowski, M. Gora, M. Szkulmowski, I. Gorczynska, D. Szlag, S. Marcos, A. Kowalczyk, and M. Wojtkowski, “Anterior segment imaging with Spectral OCT system using a high-speed CMOS camera,” Opt. Express 17(6), 4842–4858 (2009). [PubMed]
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- B. J. Kaluzny, W. Fojt, A. Szkulmowska, T. Bajraszewski, M. Wojtkowski, and A. Kowalczyk, “Spectral optical coherence tomography in video-rate and 3D imaging of contact lens wear,” Optom. Vis. Sci. 84(12), 1104–1109 (2007). [PubMed]
- B. J. Kałuzny, J. J. Kaluzny, A. Szkulmowska, I. Gorczyńska, M. Szkulmowski, T. Bajraszewski, P. Targowski, and A. Kowalczyk, “Spectral optical coherence tomography: a new imaging technique in contact lens practice,” Ophthalmic Physiol. Opt. 26(2), 127–132 (2006). [PubMed]
- M. Wojtkowski, V. Srinivasan, J. G. Fujimoto, T. Ko, J. S. Schuman, A. Kowalczyk, and J. S. Duker, “Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography,” Ophthalmology 112(10), 1734–1746 (2005). [PubMed]
- J. J. Kaluzny, M. Wojtkowski, and A. Kowalczyk, “Imaging of the anterior segment of the eye by spectral optical coherence tomography,” Opt. Appl. 32, 581–589 (2002).
- M. Wojtkowski, R. Leitgeb, A. Kowalczyk, T. Bajraszewski, and A. F. Fercher, “In vivo human retinal imaging by Fourier domain optical coherence tomography,” J. Biomed. Opt. 7(3), 457–463 (2002). [PubMed]
- A. C. Cheng, S. K. Rao, S. Lau, C. K. Leung, and D. S. Lam, “Central corneal thickness measurements by ultrasound, Orbscan II, and Visante OCT after LASIK for myopia,” J. Refract. Surg. 24(4), 361–365 (2008). [PubMed]
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- A. C. Cheng, S. K. Rao, S. Lau, C. K. Leung, and D. S. Lam, “Central corneal thickness measurements by ultrasound, Orbscan II, and Visante OCT after LASIK for myopia,” J. Refract. Surg. 24(4), 361–365 (2008). [PubMed]
- R. Leitgeb, C. K. Hitzenberger, and A. F. Fercher, “Performance of fourier domain vs. time domain optical coherence tomography,” Opt. Express 11(8), 889–894 (2003). [PubMed]
- M. Wojtkowski, R. Leitgeb, A. Kowalczyk, T. Bajraszewski, and A. F. Fercher, “In vivo human retinal imaging by Fourier domain optical coherence tomography,” J. Biomed. Opt. 7(3), 457–463 (2002). [PubMed]
- U. Schmidt-Erfurth, R. A. Leitgeb, S. Michels, B. Povazay, S. Sacu, B. Hermann, C. Ahlers, H. Sattmann, C. Scholda, A. F. Fercher, and W. Drexler, “Three-dimensional ultrahigh-resolution optical coherence tomography of macular diseases,” Invest. Ophthalmol. Vis. Sci. 46(9), 3393–3402 (2005). [PubMed]
- A. C. Cheng, S. K. Rao, S. Lau, C. K. Leung, and D. S. Lam, “Central corneal thickness measurements by ultrasound, Orbscan II, and Visante OCT after LASIK for myopia,” J. Refract. Surg. 24(4), 361–365 (2008). [PubMed]
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- M. Yamanari, S. Makita, and Y. Yasuno, “Polarization-sensitive swept-source optical coherence tomography with continuous source polarization modulation,” Opt. Express 16(8), 5892–5906 (2008). [PubMed]
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J. Opt. Soc. Am. A
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Science
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- D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991). [PubMed]
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