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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 18 — Aug. 31, 2009
  • pp: 15679–15684

STED microscopy with a MHz pulsed stimulated-Raman-scattering source

Brian R Rankin and Stefan W Hell  »View Author Affiliations

Optics Express, Vol. 17, Issue 18, pp. 15679-15684 (2009)

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We perform stimulated emission depletion (STED) microscopy with a novel light source consisting of a fiber-amplified, frequency doubled laser operating with a 1 MHz repetition rate and a 530 nm output coupled into a standard single mode fiber to produce a tunable spectrum of discrete peaks via stimulated Raman scattering (SRS). Using peaks at 585, 600, and 616 nm as STED light we perform STED microscopy with resolution down to 20-30 nm. The nanosecond pulsed light source should prove valuable for all forms of microscopy requiring both brilliance and multiple wavelengths in the visible range.

© 2009 OSA

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
(290.5910) Scattering : Scattering, stimulated Raman

ToC Category:

Original Manuscript: June 26, 2009
Revised Manuscript: August 10, 2009
Manuscript Accepted: August 11, 2009
Published: August 20, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Brian R Rankin and Stefan W Hell, "STED microscopy with a MHz pulsed stimulated-Raman-scattering source," Opt. Express 17, 15679-15684 (2009)

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  1. S. W. Hell and J. Wichmann, “Breaking the Diffraction Resolution Limit by Stimulated-Emission - Stimulated-Emission-Depletion Fluorescence Microscopy,” Opt. Lett. 19(11), 780–782 (1994). [CrossRef] [PubMed]
  2. B. Hein, K. I. Willig, and S. W. Hell, “Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell,” Proc. Natl. Acad. Sci. U.S.A. 105(38), 14271–14276 (2008). [CrossRef] [PubMed]
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