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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 2 — Jan. 19, 2009
  • pp: 1003–1015

Interferometric 3D tracking of several particles in a scanning laser focus

Michael Speidel, Lars Friedrich, and Alexander Rohrbach  »View Author Affiliations

Optics Express, Vol. 17, Issue 2, pp. 1003-1015 (2009)

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High-Speed tracking of several particles allows measuring dynamic long-range interactions relevant to biotechnology and colloidal physics. In this paper we extend the successful technique of 3D back-focal plane interferometry to oscillating laser beams and show that two or more particles can be trapped and tracked with a precision of a few nanometers in all three dimensions. The tracking rate of several kHz is only limited by the scan speed of the beam steering device. Several tests proof the linearity and orthogonality of our detection scheme, which is of interest to optical tweezing applications and various metrologies. As an example we show the position cross-correlations of three diffusing particles in a scanning line optical trap.

© 2009 Optical Society of America

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(140.7010) Lasers and laser optics : Laser trapping
(260.3160) Physical optics : Interference
(290.0290) Scattering : Scattering

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: December 2, 2008
Revised Manuscript: January 12, 2009
Manuscript Accepted: January 12, 2009
Published: January 13, 2009

Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics

Michael Speidel, Lars Friedrich, and Alexander Rohrbach, "Interferometric 3D tracking of several particles in a scanning laser focus," Opt. Express 17, 1003-1015 (2009)

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