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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 2 — Jan. 19, 2009
  • pp: 984–990

Spectral characterization of the voltage-sensitive dye di-4-ANEPPDHQ applied to probing live primary and immortalized neurons

Yu Wang, Gaoshan Jing, Susan Perry, Filbert Bartoli, and Svetlana Tatic-Lucic  »View Author Affiliations


Optics Express, Vol. 17, Issue 2, pp. 984-990 (2009)
http://dx.doi.org/10.1364/OE.17.000984


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Abstract

Spectral properties of a recently developed voltage-sensitive dye, di-4-ANEPPDHQ, were characterized as the dye was dissolved in the solvent dimethyl sulfoxide as the stock solution, in Hank's buffered salt solution as the staining solution, and bound to the plasma membrane of primary rat hippocampal neurons and immortalized mouse hypothalamic neurons (GT1-7) in vitro. Their dependence on the local chemical and electrical environment of dye molecules was determined. The excitation and emission peaks are 479nm and 570 nm for the stained primary neurons, and 476nm and 585 nm for the stained immortalized neurons. The excitation and emission bands of the stained GT1-7 neurons, defined as 50% peak intensity, are 429-516nm and 544-648nm, respectively.

© 2009 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(300.6170) Spectroscopy : Spectra

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 6, 2008
Revised Manuscript: December 31, 2008
Manuscript Accepted: January 12, 2009
Published: January 13, 2009

Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Yu Wang, Gaoshan Jing, Susan Perry, Filbert Bartoli, and Svetlana Tatic-Lucic, "Spectral characterization of the voltage-sensitive dye di-4-ANEPPDHQ applied to probing live primary and immortalized neurons," Opt. Express 17, 984-990 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-2-984


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