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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 21 — Oct. 12, 2009
  • pp: 18408–18418

Hybrid shear force feedback/scanning quantitative phase microscopy applied to subsurface imaging

Kert Edward, Faramarz Farahi, and Robert Hocken  »View Author Affiliations


Optics Express, Vol. 17, Issue 21, pp. 18408-18418 (2009)
http://dx.doi.org/10.1364/OE.17.018408


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Abstract

Quantitative phase microscopy allows for the study of the surface morphology and dynamics of transparent biological specimens. Although phase data often contains coupled subsurface information, decoupling the surface and subsurface components is often very difficult or impossible. We hereby present a simple procedure which exploits simultaneous obtained quantitative phase and shear-force feedback topography data to extract subsurface sample information. Our results reveal subsurface features in fabricated samples and fish erythrocytes.

© 2009 OSA

OCIS Codes
(100.2960) Image processing : Image analysis
(110.4190) Imaging systems : Multiple imaging
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.1530) Medical optics and biotechnology : Cell analysis
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.5810) Microscopy : Scanning microscopy
(100.3175) Image processing : Interferometric imaging

ToC Category:
Microscopy

History
Original Manuscript: June 1, 2009
Revised Manuscript: July 20, 2009
Manuscript Accepted: August 11, 2009
Published: September 28, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Kert Edward, Faramarz Farahi, and Robert Hocken, "Hybrid shear force feedback/scanning quantitative phase microscopy applied to subsurface imaging," Opt. Express 17, 18408-18418 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-21-18408


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