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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 21 — Oct. 12, 2009
  • pp: 19085–19092

Optical-fiber-microsphere for remote fluorescence correlation spectroscopy

Heykel Aouani, Frédérique Deiss, Jérôme Wenger, Patrick Ferrand, Neso Sojic, and Hervé Rigneault  »View Author Affiliations


Optics Express, Vol. 17, Issue 21, pp. 19085-19092 (2009)
http://dx.doi.org/10.1364/OE.17.019085


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Abstract

Fluorescence correlation spectroscopy (FCS) is a versatile method that would greatly benefit to remote optical-fiber fluorescence sensors. However, the current state-of-the-art struggles with high background and low detection sensitivities that prevent the extension of fiber-based FCS down to the single-molecule level. Here we report the use of an optical fiber combined with a latex microsphere to perform FCS analysis. The sensitivity of the technique is demonstrated at the single molecule level thanks to a photonic nanojet effect. This offers new opportunities for reducing the bulky microscope setup and extending FCS to remote or in vivo applications.

© 2009 Optical Society of America

OCIS Codes
(120.0280) Instrumentation, measurement, and metrology : Remote sensing and sensors
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(350.3950) Other areas of optics : Micro-optics

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: August 27, 2009
Manuscript Accepted: October 5, 2009
Published: October 8, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Heykel Aouani, Frédérique Deiss, Jérôme Wenger, Patrick Ferrand, Neso Sojic, and Hervé Rigneault, "Optical-fiber-microsphere for remote fluorescence correlation spectroscopy," Opt. Express 17, 19085-19092 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-21-19085


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References

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