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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 22 — Oct. 26, 2009
  • pp: 19486–19500

Simultaneous dual-band optical coherence tomography in the spectral domain for high resolution in vivo imaging

Peter Cimalla, Julia Walther, Mirko Mehner, Maximiliano Cuevas, and Edmund Koch  »View Author Affiliations


Optics Express, Vol. 17, Issue 22, pp. 19486-19500 (2009)
http://dx.doi.org/10.1364/OE.17.019486


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Abstract

Optical coherence tomography (OCT) in the spectral domain is demonstrated simultaneously at two wavelength bands centered at 800 nm and 1250 nm. A novel commercial supercontinuum laser is applied as a single low coherence broadband light source. The emission spectrum of the source is shaped by optical and spatial filtering in order to achieve an adequate double peak spectrum containing the wavelength bands 700 - 900 nm and 1100 - 1400 nm for dual-band OCT imaging and thus reducing the radiation exposure of the sample. Each wavelength band is analyzed with an individual spectrometer at an A-scan rate of about 12 kHz which enables real-time imaging for the examination of moving samples. A common path optical setup optimized for both spectral regions with a separate single fiber-based scanning unit was realized which facilitates flexible handling and easy access to the measurement area. The free-space axial resolutions were measured to be less than 4.5 µm and 7 µm at 800 nm and 1250 nm, respectively. Three-dimensional imaging ten times faster than previously reported with a signal-to-noise-ratio of above 90 dB is achieved simultaneously in both wavelength bands. Spectral domain dual-band OCT combines real-time imaging with high resolution at 800 nm and enhanced penetration depth at 1250 nm and therefore provides a well suited tool for in vivo vasodynamic measurements. Further, spatially resolved spectral features of the sample are obtained by means of comparing the backscattering properties at two different wavelength bands. The ability of dual-band OCT to enhance tissue contrast and the sensitivity of this imaging modality to wavelength-dependent sample birefringence is demonstrated.

© 2009 OSA

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 17, 2009
Revised Manuscript: September 16, 2009
Manuscript Accepted: October 8, 2009
Published: October 13, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Peter Cimalla, Julia Walther, Mirko Mehner, Maximiliano Cuevas, and Edmund Koch, "Simultaneous dual-band optical coherence tomography in the spectral domain for high resolution in vivo imaging," Opt. Express 17, 19486-19500 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-22-19486


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