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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 22 — Oct. 26, 2009
  • pp: 20039–20050

Long period grating working in transition mode as promising technological platform for label-free biosensing

P. Pilla, P. Foglia Manzillo, V. Malachovska, A. Buosciolo, S. Campopiano, A. Cutolo, L. Ambrosio, M. Giordano, and A. Cusano  »View Author Affiliations

Optics Express, Vol. 17, Issue 22, pp. 20039-20050 (2009)

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We present the development of a platform for label-free biosensing based on overlayered Long Period Gratings (LPGs) working in transition mode. Nano-scale layers of Polystyrene (PS) with different thicknesses were deposited onto the same LPG to test the performances of the device in different working points of its modified sensitivity characteristic. Adsorption dynamic of biotinylated bovine serum albumin (BBSA) onto the PS overlays was on-line monitored as well as a subsequent streptavidin (SA) binding dynamic on the biotinylated sites of the protein ad-layer. Experimental results show that overlayered LPGs are among the most sensitive refractive index transducers to be employed in label-free biochemical detection and that wide margins of further optimization exist.

© 2009 OSA

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(240.0310) Optics at surfaces : Thin films
(350.2770) Other areas of optics : Gratings
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:
Fiber Optics and Optical Communications

Original Manuscript: June 8, 2009
Revised Manuscript: July 10, 2009
Manuscript Accepted: July 11, 2009
Published: October 20, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

P. Pilla, P. Foglia Manzillo, V. Malachovska, A. Buosciolo, S. Campopiano, A. Cutolo, L. Ambrosio, M. Giordano, and A. Cusano, "Long period grating working in transition mode as promising technological platform for label-free biosensing," Opt. Express 17, 20039-20050 (2009)

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